冷等离子体处理玉米醇溶蛋白-原花青素复合纳米颗粒的制备、表征与性能研究  

Preparation,Characterization,and Performance of Cold Plasma-Treated Zein-Proanthocyanidin Composite Nanoparticles

作　　者：李玺 张文 尚秋 杨芳 LI Xi;ZHANG Wen;SHANG Qiu;YANG Fang(Wuhan Jinyintan Hospital,Tongji Medical College of Huazhong University of Science and Technology,Hubei Clinical Research Center for Infectious Diseases,Wuhan Research Center for Communicable Disease Diagnosis and Treatment,Chinese Academy of Medical Sciences,Joint Laboratory of Infectious Diseases and Health,Wuhan Institute of Virology and Wuhan Jinyintan Hospital,Chinese Academy of Sciences,Wuhan 430023,China;School of Environmental Ecology and Biological Engineering,Wuhan Institute of Technology,Wuhan 430205,China)

机构地区：[1]华中科技大学同济医学院附属武汉金银潭医院(感染科)湖北省传染病临床医学研究中心中国医学科学院武汉传染性疾病诊治研究中心中国科学院武汉病毒研究所&武汉市金银潭医院感染性疾病与健康联合实验室,湖北武汉430023 [2]武汉工程大学环境生态与生物工程学院,湖北武汉430205

出　　处：《化学与生物工程》2024年第10期50-57,共8页Chemistry & Bioengineering

基　　金：湖北省大学生创新创业训练计划项目(S202310490021);武汉工程大学校长基金资助项目(XZJJ2023049);国家自然科学基金青年科学基金项目(31701604)。

摘　　要：采用不同载气的冷等离子体处理玉米醇溶蛋白(zein)后包封原花青素(PC),制备复合纳米颗粒,并对其进行表征和性能研究。结果表明,经冷等离子体处理后的玉米醇溶蛋白纳米颗粒的粒径和多分散系数PDI无显著变化,而Zeta电位的绝对值显著降低;以O_(2)+Ar为载气的冷等离子体处理的复合纳米颗粒PZOA的包封率达到90.59%,显著高于以NH_(3)为载气的冷等离子体处理的复合纳米颗粒PZN(62.66%);圆二色谱分析表明,包封PC后的复合纳米颗粒的二级结构发生了变化,表明PC的加入影响了玉米醇溶蛋白的结构特征;包封PC后的复合纳米颗粒的抗氧化活性显著提高,自由基清除率由未包封的54.43%~56.03%上升至88.41%~90.29%。We prepared the zein-proanthocyanidin composite nanoparticles by encapsulating proanthocyanidins(PC)with zein treated by cold plasma with different carrier gases,characterized their structures,and studied their performances.The results show that,the particle size and polydispersity index(PDI)of zein nanoparticles treated by cold plasma have a negligible change,while the absolute value of Zeta potential decreases significantly.The encapsulation rate of PZOA treated by cold plasma with O_(2)+Ar as a carrier gas reaches 90.59%,significantly higher than that of PZN treated by cold plasma with NH_(3) as a carrier gas(62.66%).Circular dichroism analysis shows that the secondary structure of the composite nanoparticles encapsulated with PC changes,indicating that the addition of PC affects the structural characteristics of zein.The antioxidant activity of the composite nanoparticles encapsulated with PC improves significantly,and the free radical scavenging rate increases from 54.43%-56.03%to 88.41%-90.29%.

关 键 词：冷等离子体 玉米醇溶蛋白 包封 原花青素 纳米颗粒 制备 表征 性能 

分 类 号：TQ460[化学工程—制药化工] R944.9[医药卫生—药剂学]