Systemic analysis of metabolome reconfiguration in Arabidopsis after abiotic stressors uncovers metabolites that modulate defense against pathogens  

作　　者：Antoni Garcia-Molina Victoria Pastor 

机构地区：[1]Centre for Research in Agricultural Genomics(CRAG)CSIC-IRTA-UAB-UB,C/Vall Moronta,Edifici CRAG,08193 Bellaterra(Cerdanyola del Valles),Barcelona,Spain [2]Department of Biology,Biochemistry,and Natural Sciences,School of Technology and Experimental Sciences,Universitat Jaume I,12006 Castellode la Plana,Spain

出　　处：《Plant Communications》2024年第1期55-68,共14页植物通讯（英文）

基　　金：funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement no.101028809 (AG-M);from project RTI2018-094350-B-C33 funded by the Spanish Ministry for Science,Innovation and Universities (VP);from grants SEV-2015-0533 and CEX2019-000902-S funded by MCIN/AEI/10.13039/501100011033;from the CERCA Programme/Generalitat de Catalunya.

摘　　要：Understanding plant immune responses is complex because of the high interdependence among biological processes in homeostatic networks.Hence,the integration of environmental cues causes network rewiring that interferes with defense responses.Similarly,plants retain molecular signatures configured under abiotic stress periods to rapidly respond to recurrent stress,and these can alter immunity.Metabolome changes imposed by abiotic stressors are persistent,although their impact on defense remains to be clar-ified.In this study,we profiled metabolomes of Arabidopsis plants under several abiotic stress treatments applied individually or simultaneously to capture temporal trajectories in metabolite composition during adverse conditions and recovery.Further systemic analysis was performed to address the relevance of me-tabolome changes and extract central features to be tested in planta.Our results demonstrate irreversibility in major fractions of metabolome changes as a general pattern in response to abiotic stress periods.Func-tionalanalysisofmetabolomesandco-abundancenetworkspoints toconvergence inthereconfigurationof organic acid and secondary metabolite metabolism.Arabidopsis mutant lines for components related to these metabolic pathways showed altered defense capacities against different pathogens.Collectively,our data suggest that sustained metabolome changes configured in adverse environments can act as mod-ulators of immune responses and provide evidence for a new layer of regulation in plant defense.

关 键 词：abiotic stress ARABIDOPSIS biotic stress combinatorial stress metabolomics systems biology 

分 类 号：Q94[生物学—植物学]