3D genome structural variations play important roles in regulating seed oil content of Brassica napus  

作　　者：Libin Zhang Lin Liu Huaixin Li Jianjie He Hongbo Chao Shuxiang Yan Yontai Yin Weiguo Zhao Maoteng Li 

机构地区：[1]College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China [2]Wuhan Frasergen Bioinformatics Co.,Ltd.,Wuhan 430075,China [3]Key Laboratory of Molecular Biophysics of the Ministry of Education,Hubei Bioinformatics and Molecular Imaging Key Laboratory,Wuhan 430074,China

出　　处：《Plant Communications》2024年第1期101-115,共15页植物通讯（英文）

基　　金：supported by the National Key Research and Development Program of China (2022YFD1200402);the National Natural Science Foundation of China (32272067 and 32072098).

摘　　要：Dissecting the complex regulatory mechanism of seed oil content(SOC)is one of the main research goals in Brassica napus.Increasing evidence suggests that genome architecture is linked to multiple biological functions.However,the effect of genome architecture on SOC regulation remains unclear.Here,we used high-throughput chromatin conformation capture to characterize differences in the three-dimen-sional(3D)landscape of genome architecture of seeds from two B.napus lines,N53-2(with high SOC)and Ken-C8(with low SOC).Bioinformatics analysis demonstrated that differentially accessible regions and differentially expressed genes between N53-2 and Ken-C8 were preferentially enriched in regions with quantitative trait loci(QTLs)/associated genomic regions(AGRs)for SOC.A multi-omics analysis demonstrated that expression of SOC-related genes was tightly correlated with genome structural varia-tions in QTLs/AGRs of B.napus.The candidate gene BnaA09g48250D,which showed structural variation in a QTL/AGR on chrA09,was identified byfine-mapping of a KN double-haploid population derived from hybridization of N53-2 and Ken-C8.Overexpression and knockout of BnaA09g48250D led to significant in-creases and decreases in SOC,respectively,in the transgenic lines.Taken together,our results reveal the 3D genome architecture of B.napus seeds and the roles of genome structural variations in SOC regulation,enriching our understanding of the molecular mechanisms of SOC regulation from the perspective of spatial chromatin structure.

关 键 词：Brassica napus seed oil content Hi-C QTLfine-mapping genome structural variation 

分 类 号：S565.4[农业科学—作物学]