Natural variations of HvSRN1 modulate the spike rachis node number in barley  

作　　者：Chaofeng Fan Dongdong Xu Chunchao Wang Zhaoyan Chen Tingyu Dou Dandan Qin Aikui Guo Meng Zhao Honghong Pei Mengwei Zhao Renxu Zhang Ke Wang Jing Zhang Zhongfu Ni Ganggang Guo 

机构地区：[1]Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization(MARA),The National Key Facility for Crop Gene Resources and Genetic Improvement,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences(ICS-CAAS),Beijing 100081,China [2]Hainan Yazhou Bay Seed Laboratory,Sanya 572025,China [3]Institute of Industrial Crops,Shandong Academy of Agricultural Sciences,Jinan 250100,China [4]Key Laboratory of Crop Heterosis and Utilization,Beijing Key Laboratory of Crop Genetic Improvement,China Agricultural University,Beijing 100193,China [5]Key Laboratory for Crop Molecular Breeding of Ministry of Agriculture and Rural Affairs,Institute of Food Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,China

出　　处：《Plant Communications》2024年第1期148-163,共16页植物通讯（英文）

基　　金：supported by the National Key R&D Program of China (2018YFD1000706);Hainan Yazhou Bay Seed Laboratory (B21Y10214);the National Natural Science Foundation of China (no.31771774);the Agricultural Science and Technology Innovation Program,and the China Agricultural Research System (CARS-05).

摘　　要：Grain number,one of the major determinants of yield in Triticeae crops,is largely determined by spikelet number and spike rachis node number(SRN).Here,we identified three quantitative trait loci(QTLs)for SRN using 145 recombinant inbred lines derived from a barley R90/1815D cross.qSRN1,the major-effect QTL,was mapped to chromosome 2H and explained up to 38.77%of SRN variation.Map-based cloning revealed that qSRN1 encodes the RAWUL domain-containing protein HvSRN1.Further analysis revealed that two key SNPs in the HvSRN1 promoter region(-2 kb upstream of the transcription start site)affect the transcript level of HvSRN1 and contribute to variation in SRN.Similar to its orthologous proteins OsLAX2 and ZmBA2,HvSRN1 showed protein–protein interactions with HvLAX1,suggesting that the LAX2–LAX1 model for spike morphology regulation may be conserved in Poaceae crops.CRISPR-Cas9-induced HvSRN1 mutants showed reduced SRN but increased grain size and weight,demonstrating a trade-off effect.Our results shed light on the role of HvSRN1 variation in regulating the balance between grain number and weight in barley.

关 键 词：BARLEY FINE-MAPPING grain number kernel weight 

分 类 号：S512.3[农业科学—作物学]