A chromosome-level genome assembly of anesthetic drug–producing Anisodus acutangulus provides insights into its evolution and the biosynthesis of tropane alkaloids  

作　　者：Wei Zhou Can Wang Xiaolong Hao Fei Chen Qikai Huang Tingyao Liu Jiang Xu Shuai Guo Baosheng Liao Zhixiang Liu Yue Feng Yao Wang Pan Liao Jiayu Xue Min Shi Itay Maoz Guoyin Kai 

机构地区：[1]Zhejiang Provincial International S&T Cooperation Base for Active Ingredients of Medicinal and Edible Plants and Health,Zhejiang Provincial Key TCM Laboratory for Chinese Resource Innovation and Transformation,School of Pharmaceutical Sciences,Jinhua Academy,Zhejiang Chinese Medical University,Hangzhou,Zhejiang 310053,China [2]Sanya Nanfan Research Institute from Hainan University,Hainan Yazhou Bay Seed Laboratory,Sanya 572025,China [3]Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China [4]Department of Biology,Hong Kong Baptist University,Kowloon Tong,Hong Kong,China [5]College of Horticulture,Academy for Advanced Interdisciplinary Studies,Nanjing Agricultural University,Nanjing 210095,China [6]Department of Postharvest Science,Agricultural Research Organization,Volcani Center,P.O.Box 15159,HaMaccabim Road 68,Rishon LeZion 7505101,Israel

出　　处：《Plant Communications》2024年第1期231-244,共14页植物通讯（英文）

基　　金：supported by the National"Ten-Thousand Talents Program"for Leading Talents of Science and Technology Innovation in China,the National Key R&D Program of China (2018YFC1706203);the National Young Qihuang Scholars Training Program,the National Natural Science Foundation of China (82003888,82073963,81522049,82003889);a Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural Varieties (2021C02074-3);the"Sannongjiufang"Research Joint Project of Zhejiang Province (2023SNJF029);the Zhejiang Provincial Natural Science Foundation of China (LY20H280008);Research Projects of Zhejiang Chinese Medical University (2021JKZKTS011A,2021JKZDZC06,2020010,and YB20023).

摘　　要：Tropane alkaloids(TAs),which are anticholinergic agents,are an essential class of natural compounds,and there is a growing demand for TAs with anesthetic,analgesic,and spasmolytic effects.Anisodus acutan-gulus(Solanaceae)is a TA-producing plant that was used as an anesthetic in ancient China.In this study,we assembled a high-quality,chromosome-scale genome of A.acutangulus with a contig N50 of 7.4 Mb.A recent whole-genome duplication occurred in A.acutangulus after its divergence from other Solanaceae species,which resulted in the duplication of ADC1 and UGT genes involved in TA biosynthesis.The catalytic activities of H6H enzymes were determined for three Solanaceae plants.On the basis of evolution and co-expressed genes,AaWRKY11 was selected for further analyses,which revealed that its encoded tran-scription factor promotes TA biosynthesis by activating AaH6H1 expression.Thesefindings provide useful insights into genome evolution related to TA biosynthesis and have potential implications for genetic manipulation of TA-producing plants.

关 键 词：Anisodus acutangulus tropane alkaloid BIOSYNTHESIS hyoscyamine 6 b-hydroxylase AaWRKY11 

分 类 号：Q94[生物学—植物学]