The new era of cardiovascular research:revolutionizing cardiovascular research with 3D models in a dish  

作　　者：Yuan Yang Hao Yang Fedir N.Kiskin Joe Z.Zhang 

机构地区：[1]Institute of Neurological and Psychiatric Disorders,Shenzhen Bay Laboratory,Shenzhen,Guangdong Province,China [2]Institute of Neurological and Psychiatric Disorders,Shenzhen Bay Laboratory,Shenzhen 518132,Guangdong Province,China

出　　处：《Medical Review》2024年第1期68-85,共18页医学评论（英文）

基　　金：funded by National Natural Science Foundation of China General Program(Grant#82370311);Guangdong Province International Science and Technology Cooperation Research Project(Grant#2023A0505050088)。

摘　　要：Cardiovascular research has heavily relied on studies using patient samples and animal models.However,patient studies often miss the data from the crucial early stage of cardiovascular diseases,as obtaining primary tissues at this stage is impracticable.Transgenic animal models can offer some insights into disease mechanisms,although they usually do not fully recapitulate the phenotype of cardiovascular diseases and their progression.In recent years,a promising breakthrough has emerged in the form of in vitro three-dimensional(3D)cardiovascular models utilizing human pluripotent stem cells.These innovative models recreate the intricate 3D structure of the human heart and vessels within a controlled environment.This advancement is pivotal as it addresses the existing gaps in cardiovascular research,allowing scientists to study different stages of cardiovascular diseases and specific drug responses using human-origin models.In this review,we first outline various approaches employed to generate these models.We then comprehensively discuss their applications in studying cardiovascular diseases by providing insights into molecular and cellular changes associated with cardiovascular conditions.Moreover,we highlight the potential of these 3D models serving as a platform for drug testing to assess drug efficacy and safety.Despite their immense potential,challenges persist,particularly in maintaining the complex structure of 3D heart and vessel models and ensuring their function is comparable to real organs.However,overcoming these challenges could revolutionize cardiovascular research.It has the potential to offer comprehensive mechanistic insights into human-specific disease processes,ultimately expediting the development of personalized therapies.

关 键 词：pluripotent stem cells three-dimensional(3D)models cardiac microtissues cardiac organoids vessel organoids organ-on-a-chip 

分 类 号：R54[医药卫生—心血管疾病]