Adaptive multiscale microscope with fast zooming,extended working distance,and large field of view  

作　　者：Yi Zheng Xin Wang Zhao Jiang Jinbo Xu Rongying Yuan Youran Zhao Haoran Zhang Chao Liu Qionghua Wang 

机构地区：[1]School of Instrumentation and Optoelectronic Engineering,Beihang University,Beijing 100191,China [2]State Key Laboratory of Virtual Reality Technology and Systems,Beihang University,Beijing 100191,China

出　　处：《Light(Advanced Manufacturing)》2024年第1期60-72,共13页光（先进制造）（英文）

基　　金：supported by the National Natural Science Foundation of China under Grant Nos.61927809 and 62175006;Beijing Natural Science Foundation under Grant No.4222069;Shenzhen Science and Technology Innovation Commission under Grant No.JCYJ20220818100413030.

摘　　要：The field-of-view(FOV),depth of field,and resolution of conventional microscopes are constrained by each other;therefore,a zoom function is required.Traditional zoom methods lose real-time performance and have limited information throughput,severely limiting their application,especially in three-dimensional dynamic imaging and large-amount or large-size sample scanning.Here,an adaptive multiscale(AMS)imaging mechanism combining the benefits of liquid lenses and multiscale imaging techniques is proposed to realize the functions of fast zooming,wide working distance(WD)range and large FOV on a self-developed AMS microscope.The design principles were revealed.Moreover,a nonuniform-distortion-correction algorithm and a composite patching algorithm were designed to improve image quality.The continuous tunable magnification range of the AMS microscope is from 9×to 18×,with the corresponding FOV diameters and resolution ranging from 2.31 to 0.98 mm and from 161 to 287 line-pairs/mm,respectively.The extended WD range is 0.8 mm and the zoom response time is 38 ms.Experiments demonstrated the advantages of the proposed microscope in pathological sample scanning,thick-sample imaging,microfluidic process monitoring,and the observation of living microorganisms.The proposed microscope is the first step towards zoom multiscale imaging technology and is expected to be applied in life sciences,medical diagnosis,and industrial detection.

关 键 词：MICROSCOPE Multiscale Liquid lens ZOOM 

分 类 号：TH742[机械工程—光学工程]