Combined impacts of bioaugmentation and vermiremediation on crude oil-contaminated soil: Mitigating strategies for prospective environmental management  

作　　者：Fuad Ameen Ali A.Al-Homaidan 

机构地区：[1]Department of Botany&Microbiology,College of Science,King Saud University,Riyadh,11451,Saudi Arabia

出　　处：《Emerging Contaminants》2024年第2期123-134,共12页新兴污染物（英文）

基　　金：This project was funded by the National Plan for Science,Technology and Innovation(MAARIFAH),King Abdulaziz City for Science and Technology,Kingdom of Saudi Arabia,Award Number(2-17-01-001-0063).

摘　　要：This study aimed to address the remediation of crude oil-contaminated soil by employing four different factorial setups:I-used the earthworms(Eudrilus eugeniae)for vermiremediation,II–utilized the bacteria(Pseudomonas aeruginosa FA-7)for bioaugmentation,III–combined earthworms+bacteria to perform vermiremediation+bioaugmentation,and IV–served as abiotic control.Further,garden soil weighing 1 kg and 50 g of desiccated cow dung were introduced into the experimental groups and abiotic controls.Subsequently,different quantities of crude oil ranging from 5 to 20 mL were introduced.Based on the results,from 5 mL of oil-contaminated soil,the factorial setup-III removed the maximum proportion of total petroleum hydrocarbons(TPH)in V+B1(58.06%).The abiotic control treatments AC1(1.11%)exhibited the lowest TPH removal rate.The bioremediated soil had significantly(P<0.05)different physicochemical properties than the initial crude oil contaminated soil.For instance,earthworm and microbial action resulted in considerable reductions in pH(4.72%),total organic carbon(44.03%),total nitrogen(42.42%),total phosphorus(52.17%),and total potassium(22.64%)by the end of the day.On the 60th day of factorial setup-III,the bacterial population(386.470±0.432 CFU×10^(6) g^(−1)),actinomycetes(188.043±0.246 CFU×10^(8) g^(−1)),and fungi(16.603±0.202 CFU×10^(5) g^(−1))were increased.The germination rates of Trigonella foenum-graecum seeds were increased by 86.6%in factorial setup III(V+B1)when compared to other factorial setups.Hence,the incorporation of vermiremediation and bioaugmentation provides a distinctive platform for effective oil-contaminated soil remediation.

关 键 词：Crude oil Eudrilus eugeniae Pseudomonas aeruginosa FA-7 BIOREMEDIATION Seed germination 

分 类 号：S15[农业科学—土壤学]