肠道菌群代谢产物乙酸介导短期高脂饮食诱导米色脂肪组织产热的研究  

Short-Term High-Fat Diet Promotes Beige Adipose Tissue Thermogenesis Through Acetate Secreted By Gut Microbiota

作　　者：向李倩 罗岩 孙翠 胡凯悦 钱莉 刘煜 Xiang Liqian;Luo Yan;Sun Cui;Hu Kaiyue;Qian Li;Liu Yu(Department of Endocrinology,Sir Run Run Hospital,Nanjing Medical University,Nanjing 211166,China)

机构地区：[1]南京医科大学附属逸夫医院内分泌科,南京211166

出　　处：《成都医学院学报》2024年第5期745-750,共6页Journal of Chengdu Medical College

基　　金：国家自然科学基金项目(No:82300975);南京市卫生健康委员会、南京市财政局卫生科技发展基金项目(No:YKK21257)。

摘　　要：目的探索短期高脂饮食对小鼠肠道菌群和米色脂肪组织产热的影响及其相关机制。方法8周龄C57BL/6雄性小鼠分别以高脂饮食或正常饲料喂养1周,测定体重、组织重量和形态学、血清甘油三酯和总胆固醇,对米色脂肪组织的基因表达谱进行RNA测序分析,并采用实时荧光定量PCR(qRT-PCR)和蛋白质印迹法进行验证;同时,采用16S rDNA检测粪便微生物群;抗生素及乙酸处理验证微生物群对短期高脂饮食诱导的米色脂肪产热的介导。结果短时间高脂饮食不改变小鼠的体重和血脂水平,但腹股沟(P=0.006)和附睾(P=0.001)白色脂肪组织的重量增加,氧化磷酸化和产热相关的基因表达上调(P<0.05);同时,粪便微生物群的多样性和丰度发生了明显变化(P<0.05),特别是短链脂肪酸产生菌的丰度增加;抗生素清除肠道菌群减弱高脂饮食对米色脂肪组织产热的促进作用;补充乙酸盐可促进米色脂肪产热及2型免疫。结论短期高脂饮食通过重塑肠道菌群促进乙酸产生,激活米色脂肪组织2型免疫及产热。Objective In this study,we investigated how a short-term high-fat diet(HFD)regulated gut microbiota and thermogenesis of beige adipose tissue in mice.Methods Eight-week-old C57BL/6 male mice were fed with either HFD or normal chow diet for 1 week.Body weight,tissue weight,morphology,and serum triglycerides and total cholesterol were determined.And the gene expression profiles of beige adipose tissue were analyzed by RNA sequencing and verified by quantitative real-time PCR(qRT-PCR)and western blot.Meanwhile,16S rDNA sequencing was used to detect fecal microbiota.Antibiotics and acetate treatments were applied to verify the mediating role of microbiota on beige adipose tissue thermogenesis induced by short-term HFD.Results A short-term HFD did not change body weight and lipid levels in mice,but increased the weight of white adipose tissue in the groin(P=0.006)and epididymis(P=0.001),and up-regulated the expression of genes related to oxidative phosphorylation and thermogenesis(P<0.05).The diversity and abundance of fecal microbiota changed significantly(P<0.05),with significant increase in the abundance of short-chain fatty acid producing bacteria.The promoting effect of HFD on beige adipose tissue thermogenesis was blunted by microbiota depletion with antibiotics.Acetate supplementation restored beige adipose tissue thermogenesis and promoted type 2 immunity in mice.Conclusion Short-term HFD promotes acetate production by reshaping the gut microbiota,activating type 2 immunity and thermogenesis in beige adipose tissue.

关 键 词：米色脂肪组织 产热 菌群 短链脂肪酸 

分 类 号：R589.2[医药卫生—内分泌]