Wheat zinc finger protein TaZF interacts with both the powdery mildew AvrPm2 protein and the corresponding wheat Pm2a immune receptor  

作　　者：Beatrice Manser Helen Zbinden Gerhard Herren Joel Steger Jonatan Isaksson Stephanie Braunlich Thomas Wicker Beat Keller 

机构地区：[1]Department of Plant and Microbial Biology,University of Zurich,Zollikerstrasse 107,8008 Zurich,Switzerland [2]Agroscope,Route de Duillier 50,1260 Nyon,Switzerland [3]BIOREBA AG,Christoph Merian-Ring 7,4153 Reinach,Switzerland

出　　处：《Plant Communications》2024年第5期25-41,共17页植物通讯（英文）

基　　金：supported by grants from the Swiss National Science Foundation (310030_204165 and 310030B_182833);by funding from the University of Zurich.

摘　　要：Plant defense responses to pathogens are induced after direct or indirect perception of effector proteins or their activity on host proteins.In fungal–plant interactions,relatively little is known about whether,in addi-tion to avirulence effectors and immune receptors,other proteins contribute to specific recognition.The nucleotide-binding leucine-rich repeat(NLR)immune receptor Pm2a in wheat recognizes the fungal pow-dery mildew effector AvrPm2.We found that the predicted wheat zincfinger TaZF interacts with both the fungal avirulence protein AvrPm2 and the wheat NLR Pm2a.We further demonstrated that the virulent AvrPm2-H2 variant does not interact with TaZF.TaZF silencing in wheat resulted in a reduction but not a loss of Pm2a-mediated powdery mildew resistance.Interaction studies showed that the leucine-rich repeat domain of Pm2a is the mediator of the interaction with TaZF.TaZF recruits both Pm2a and AvrPm2 from the cytosol to the nucleus,resulting in nuclear localization of Pm2a,TaZF,and AvrPm2 in wheat.We propose that TaZF acts as a facilitator of Pm2a-dependent AvrPm2 effector recognition.Ourfindings highlight the importance of identifying effector host targets for characterization of NLR-mediated effector recognition.

关 键 词：plant immunity plant-fungal interactions pathogen effectors nucleotide-binding leucine-rich repeat immune receptor NLR WHEAT powdery mildew 

分 类 号：S512.1[农业科学—作物学]