WRKY33 negatively regulates anthocyanin biosynthesis and cooperates with PHR1 to mediate acclimation to phosphate starvation  

作　　者：Han Tao Fei Gao Linying Li Yuqing He Xueying Zhang Mengyu Wang Jia Wei Yao Zhao Chi Zhang Qiaomei Wang Gaojie Hong 

机构地区：[1]State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Key Laboratory of Biotechnology in Plant Protection of the MOA of China and Zhejiang Province,Institute of Virology and Biotechnology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China [2]State Key Laboratory of Subtropical Silviculture,Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine,Zhejiang A&F University,Hangzhou 311300,China [3]Department of Horticulture,College of Agriculture and Biotechnology,Zhejiang University,Key Laboratory of Horticultural Plant Growth,Development and Quality Improvement,Ministry of Agriculture,Hangzhou,Zhejiang,China [4]Institute of Sericulture and Tea,Zhejiang Academy of Agricultural Sciences,Hangzhou 310000,China

出　　处：《Plant Communications》2024年第5期134-147,共14页植物通讯（英文）

基　　金：funded by the Zhejiang Provincial Natural Science Foundation of China under grant no.LR22C020003;the National Key Research and Development Program of China (2022YFD2200603);the National Natural Science Foundation of China under grant nos.32000234 and 32172593;the China Postdoctoral Science Foundation (2022M712831);funding from the State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products.

摘　　要：Anthocyanin accumulation is acknowledged as a phenotypic indicator of phosphate(Pi)starvation.However,negative regulators of this process and their molecular mechanisms remain largely unexplored.In this study,we demonstrate that WRKY33 acts as a negative regulator of phosphorus-status-dependent anthocyanin biosynthesis.WRKY33 regulates the expression of the gene encoding dihydroflavonol 4-reductase(DFR),a rate-limiting enzyme in anthocyanin production,both directly and indirectly.WRKY33 binds directly to the DFR promoter to repress its expression and also interferes with the MBW complex through interacting with PAP1 to indirectly influence DFR transcriptional activation.Under�Pi conditions,PHR1 interacts with WRKY33,and the protein level of WRKY33 decreases;the repression of DFR expression by WRKY33 is thus attenuated,leading to anthocyanin accumulation in Arabidopsis.Further genetic and biochemical assays suggest that PHR1 is also involved in regulating factors that affect WRKY33 protein turnover.Taken together,ourfindings reveal that Pi starvation represses WRKY33,a repressor of anthocyanin biosynthesis,tofinely tune anthocyanin biosynthesis.This‘‘double-negative logic’’regulation of phosphorus-status-dependent anthocyanin biosynthesis is required for the mainte-nance of plant metabolic homeostasis during acclimation to Pi starvation.

关 键 词：WRKY33 phosphate signaling PHR1 Arabidopsis thaliana anthocyanins 

分 类 号：Q94[生物学—植物学]