Two aquaporins,PIP1;1 and PIP2;1,mediate the uptake of neonicotinoid pesticides in plants  

作　　者：Qun Wan Yixin Li Jinjin Cheng Ya Wang Jing Ge Tingli Liu Liya Ma Yong Li Jianan Liu Chunli Zhou Haocong Li Xing Sun Xiaolong Chen Qing X.Li Xiangyang Yu 

机构地区：[1]Key Laboratory of Food Quality and Safety of Jiangsu Province,State Key Laboratory Breeding Base,Nanjing 210014,China [2]Institute of Food Safety and Nutrition,Jiangsu Academy of Agricultural Sciences,50 Zhongling Street,Nanjing 210014,China [3]Jiangsu Provincial Key Construction Laboratory of Special Biomass Resource Utilization,Nanjing Xiaozhuang University,3601 Hongjin Avenue,Nanjing 211171,China [4]Department of Molecular Biosciences and Bioengineering,University of Hawaii at Manoa,Honolulu,HI 96822,USA

出　　处：《Plant Communications》2024年第5期176-188,共13页植物通讯（英文）

基　　金：supported in part by the National Natural Science Foundation of China (nos.32172448 and 3177219);the Jiangsu Agricultural Science and Technology Innovation Fund (CX (21)2002);the National Key Research and Development Program (2021YFD1700803),and the USDA (HAW05032R).

摘　　要：Neonicotinoids(NEOs),a large class of organic compounds,are a type of commonly used pesticide for crop protection.Their uptake and accumulation in plants are prerequisites for their intra-and intercellular move-ments,transformation,and function.Understanding the molecular mechanisms that underpin NEO uptake by plants is crucial for effective application,which remains elusive.Here,we demonstrate that NEOs enter plant cells primarily through the transmembrane symplastic pathway and accumulate mainly in the cytosol.Two plasma membrane intrinsic proteins discovered in Brassica rapa,BraPIP1;1 and BraPIP2;1,were found to encode aquaporins(AQPs)that are highly permeable to NEOs in different plant species and facilitate NEO subcellular diffusion and accumulation.Their conserved transport function was further demonstrated in Xenopus laevis oocyte and yeast assays.BraPIP1;1 and BraPIP2;1 gene knockouts and interaction as-says suggested that their proteins can form functional heterotetramers.Assessment of the potential of mean force indicated a negative correlation between NEO uptake and the energy barrier of BraPIP1;1 chan-nels.This study shows that AQPs transport organic compounds with greater osmolarity than previously thought,providing new insight into the molecular mechanisms of organic compound uptake and facilitating innovations in systemic pesticides.

关 键 词：NEONICOTINOID AQUAPORIN Brassica rapa plasma membrane UPTAKE systemic pesticide 

分 类 号：Q94[生物学—植物学]