Multiple factors interact in editing of PPR-E+-targeted sites in maize mitochondria and plastids  

作　　者：Yong Wang Zi-Qin Huang Kai-Di Tian Hao Li Chunhui Xu Bingyujie Xia Bao-Cai Tan 

机构地区：[1]Key Laboratory of Plant Development and Environmental Adaptation Biology,Ministry of Education,School of Life Sciences,Shandong University,Qingdao 266237,China

出　　处：《Plant Communications》2024年第5期225-240,共16页植物通讯（英文）

基　　金：Dr.Tsuyoshi Nakagawa (Shimane University,Japan)for providing the pGWB vectors;supported by the National Natural Science Foundation of China (projects 32230075 and 32101640)。

摘　　要：RNA cytidine-to-uridine editing is essential for plant organellar gene expression.Pentatricopeptide repeat(PPR)-E+ proteins have been proposed to bind to target sites and recruit the cytidine deaminase AtDYW2,facilitated by AtNUWA.Here we analyze the function of ZmNUWA,ZmDYW2A,and ZmDYW2B and their re-lationships with other editing factors in maize.The zmdyw2a and zmdyw2b single mutants are normal,but the zmdyw2a::zmdyw2b and zmnuwa mutants are severely arrested in seed development.ZmNUWA,ZmDYW2A,and ZmDYW2B are dual localized in mitochondria and plastids.Loss of ZmNUWA decreases the editing at 99 mitochondrial sites and 8 plastid sites.Surprisingly,loss of ZmDYW2A:ZmDYW2B affects almost the same set of sites targeted by PPR-E+ proteins.ZmNUWA interacts with ZmDYW2A and ZmDYW2B,suggesting that ZmNUWA recruits ZmDYW2A/2B in the editing of PPR-E+-targeted sites in maize.Further protein interaction analyses show that ZmNUWA and ZmDYW2A/2B interact with ZmMORF1,ZmMORF8,ZmMORF2,and ZmMORF9 and that ZmOZ1 interacts with ZmORRM1,ZmDYW2A,ZmDYW2B,ZmMORF8,and ZmMORF9.These results suggest that the maize mitochondrial PPR-E+editosome contains PPR-E+,ZmDYW2A/2B,ZmNUWA,and ZmMORF1/8,whereas the plastid PPR-E+editosome is composed of PPR-E+,ZmDYW2A/2B,ZmNUWA,ZmMORF2/8/9,ZmORRM1,and ZmOZ1.

关 键 词：MAIZE plant organelles RNA editing ZmNUWA ZmDYW2A/2B 

分 类 号：S513[农业科学—作物学]