The MRE11–ATM–SOG1 DNA damage signaling pathway confers rice immunity to Xanthomonas oryzae  

作　　者：Zhan Xu Mingnan Qu Chuanlin Shi Hong Zhang Wu Chen Hongge Qian Zhipeng Zhang Jiehua Qiu Qian Qian Lianguang Shang 

机构地区：[1]Guangzhou City Academy of Agricultural Sciences,Key Laboratory of Biology,Genetics and Breeding,Guangzhou 510000,China [2]Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China [3]Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding,College of Agriculture,Yangzhou University,Yangzhou 225009,China [4]State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 311400,China [5]Yazhouwan National Laboratory,No.8 Huanjin Road,Yazhou District,Sanya City,Hainan Province 572024,China [6]These authors contributed equally to this article

出　　处：《Plant Communications》2024年第4期194-207,共14页植物通讯（英文）

基　　金：supported by the Guangzhou Science and Technology Planning Project (202201010790);the National Natural Science Foundation of China (32188102);the Guangdong Basic and Applied Basic Research Foundation (2023B1515020053);the Youth Innovation of Chinese Academy of Agricultural Sciences (Y20230C36);the specific research fund of The Innovation Platform for Academicians of Hainan Province (YSPTZX202303).

摘　　要：Plants are constantly exposed to microbial pathogens in the environment.One branch of innate plant immunity is mediated by cell-membrane-localized receptors,but less is known about associations between DNA damage and plant immune responses.Here,we show that rice(Oryza sativa)mesophyll cells are prone to DNA double-stranded breaks(DSBs)in response to ZJ173,a strain of Xanthomonas oryzae pv.oryzae(Xoo).The DSB signal transducer ataxia telangiectasia mutated(ATM),but not the ATM and Rad3-related branch,confers resistance against Xoo.Mechanistically,the MRE11–ATM module phosphorylates suppressor of gamma response 1(SOG1),which activates several phenylpropanoid pathway genes and prompts downstream phytoalexin biosynthesis during Xoo infection.Intriguingly,overexpression of the topoisomerase gene TOP6A3 causes a switch from the classic non-homologous end joining(NHEJ)pathway to the alternative NHEJ and homologous recombination pathways atXoo-induced DSBs.The enhanced ATM signaling of the alternative NHEJ pathway strengthens the SOG1-regulated phenylpropanoid pathway and thereby boosts Xoo-induced phytoalexin biosynthesis in TOP6A3-OE1 overexpression lines.Overall,the MRE11–ATM–SOG1 pathway serves as a prime example of plant–pathogen interactions that occur via host non-specific recognition.The function of TOP6-facilitated ATM signaling in the defense response makes it a promising target for breeding of rice germplasm that exhibits resistance to bacterial blight disease without a growth penalty.

关 键 词：Oryza sativa Xanthomonas oryzae pv.oryzae DSBs PHYTOALEXINS MRE11–ATM–SOG1 TOP6 complex 

分 类 号：Q94[生物学—植物学]