Suppression of host plant defense by bacterial small RNAs packaged in outer membrane vesicles  

作　　者：Yan Wu Sai Wang Peihong Wang Wenhan Nie Iftikhar Ahmad Panagiotis F.Sarris Gongyou Chen Bo Zhu 

机构地区：[1]Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station,Shanghai Cooperative Innovation Center for Modern Seed Industry,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai,China [2]Department of Environmental Sciences,COMSATS University Islamabad,Vehari-Campus,Vehari 61100,Pakistan [3]Department of Biology,University of Crete,71409 Heraklion,Crete,Greece [4]Shanghai Jiao Tong University Chongqing Research Institute,Shanghai,China

出　　处：《Plant Communications》2024年第4期239-250,共12页植物通讯（英文）

基　　金：supported by the National Natural Science Foundation of China (32272479,32200142);Open Project Program of State Key Laboratory of Rice Biology (20190109);Open Project Program of State Key Laboratory for Biology of Plant Diseases and Insect Pests (SKLOF202201);Zhejiang Province Ecological Environment Research and Promotion Project (2020HT0009);Shanghai Committee of Science and Technology (19390743300 and 21ZR1435500);Chongqing Natural Science Foundation (CSTB2022NSCQ-MSX0524).

摘　　要：Noncoding small RNAs(sRNAs)packaged in bacterial outer membrane vesicles(OMVs)function as novel mediators of interspecies communication.While the role of bacterial sRNAs in enhancing virulence is well established,the role of sRNAs in the interaction between OMVs from phytopathogenic bacteria and their host plants remains unclear.In this study,we employ RNA sequencing to characterize differentially pack-aged sRNAs in OMVs of the phytopathogen Xanthomonas oryzae pv.oryzicola(Xoc).Our candidate sRNA(Xosr001)was abundant in OMVs and involved in the regulation of OsJMT1 to impair host stomatal immu-nity.Xoc loads Xosr001 into OMVs,which are specifically ttransferred into the mechanical tissues of rice leaves.Xosr001 suppresses OsJMT1 transcript accumulation in vivo,leading to a reduction in MeJA accu-mulation in rice leaves.Furthermore,the application of synthesized Xosr001 sRNA to the leaves of OsJMT1-HA-OE transgenic line results in the suppression of OsJMT1 expression by Xosr001.Notably,the OsJMT1-HA-OE transgenic line exhibited attenuated stomatal immunity and disease susceptibility upon infection with DXosr001 compared to Xoc.These results suggest that Xosr001 packaged in Xoc OMVs functions to suppress stomatal immunity in rice.

关 键 词：small RNAs outer membrane vesicles Xanthomonas oryzae stomatal immunity cross-species communication 

分 类 号：Q94[生物学—植物学]