Genome editing of 30 UTR-embedded inhibitory region enables generation of gene knock-up alleles in plants  

作　　者：Hongwen Wang Dahan Zhang Mingjiang Chen Xiangbing Meng Shiwei Bai Peiyong Xin Jijun Yan Jinfang Chu Jiayang Li Hong Yu 

机构地区：[1]State Key Laboratory of Plant Genomics and National Center for Plant Gene Research,Institute of Genetics and Developmental Biology,Innovation Academy for Seed Design,Chinese Academy of Sciences,Beijing 100101,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Yazhouwan National Laboratory,Sanya 572024,China

出　　处：《Plant Communications》2024年第3期1-4,共4页植物通讯（英文）

基　　金：supported by grants from the National Key R&D Program of China (2022YFF1003403);the CAS Project for Young Scientists in Basic Research (YSBR-078);the National Natural Science Foundation of China (31788103,32122064,31801014,31900168);the Chinese Academy of Sciences (XDA24030504).

摘　　要：Dear Editor,Genome editing has revolutionized speed breeding by enabling researchers to alter the genome directly as desired(Gao,2021).The most widely used CRISPR–Cas9 technology has mainly been applied to engineer null mutations in coding sequences,with the goal of creating loss-of-function alleles.Recently,upstream regulatory elements of the promoter region and 50 upstream open reading frame(uORF)have been engineered to generate gain-of-function alleles,producing versatile cis-regulatory effects,usually through changes in gene expression levels(Rodrıguez-Leal et al.,2017;Zhang et al.,2018;Song et al.,2022).

关 键 词：UPSTREAM breeding VERSATILE 

分 类 号：Q78[生物学—分子生物学]