Haplotype-resolved chromosome-level genome of hexaploid Jerusalem artichoke provides insights into its origin,evolution,and inulin metabolism  

作　　者：Sen Wang Anqi Wang Rong Chen Dong Xu Hengchao Wang Fan Jiang Hangwei Liu Wanqiang Qian Wei Fan 

机构地区：[1]Guangdong Laboratory for Lingnan Modern Agriculture(Shenzhen Branch),Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong 518120,China [2]Guangdong Provincial Key Laboratory for Crop Germplasm Resources Preservation and Utilization,Agro-Biological Gene Research Center,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China [3]College of Agronomy,Qingdao Agricultural University,Qingdao 266109,China

出　　处：《Plant Communications》2024年第3期77-92,共16页植物通讯（英文）

基　　金：supported by the National Key R&D Program of China (2021YFC2600101);the Shenzhen Science and Technology Program (JCYJ20190814163805604 and KQTD20180411143628272);the Fund of the Key Laboratory of Shenzhen (ZDSYS20141118170111640);the Agricultural Science and Technology Innovation Program.

摘　　要：Jerusalem artichoke(Helianthus tuberosus)is a global multifunctional crop.It has wide applications in the food,health,feed,and biofuel industries and in ecological protection;it also serves as a germplasm pool for breeding of the global oil crop common sunflower(Helianthus annuus).However,biological studies of Je-rusalem artichoke have been hindered by a lack of genome sequences,and its high polyploidy and large genome size have posed challenges to genome assembly.Here,we report a 21-Gb chromosome-level as-sembly of the hexaploid Jerusalem artichoke genome,which comprises 17 homologous groups,each with 6 pseudochromosomes.We found multiple large-scale chromosome rearrangements between Jerusalem artichoke and common sunflower,and our results show that the hexaploid genome of Jerusalem artichoke was formed by a hybridization event between a tetraploid and a diploid Helianthus species,followed by chromosome doubling of the hybrid,which occurred approximately 2 million years ago.Moreover,we iden-tied more copies of actively expressed genes involved in inulin metabolism and showed that these genes may still be undergoing loss of function or sub-or neofunctionalization.These genomic resources will pro-mote further biological studies,breeding improvement,and industrial utilization of Helianthus crops.

关 键 词：Helianthus tuberosus hexaploid genome hybridization origin chromosome rearrangement inulin metabolism genes 

分 类 号：Q94[生物学—植物学]