A dephosphorylation-dependentmolecular switch for FT repression mediates flowering in Arabidopsis  

作　　者：Yu Zhang Changmei Hua Jian Xuan Kiang Lisha Shen 

机构地区：[1]Temasek Life Sciences Laboratory,1 Research Link,National University of Singapore,Singapore 117604,Singapore [2]Department of Biological Sciences,Faculty of Science,National University of Singapore,Singapore 117543,Singapore

出　　处：《Plant Communications》2024年第3期167-178,共12页植物通讯（英文）

基　　金：supported by the National Research Foundation Competitive Research Programme (NRF-CRP22-2019-0001);the Singapore Food Story R&D Programme (SFS_RND_SUFP_001_04);intramural research support from Temasek Life Sciences Laboratory.

摘　　要：The reproductive success offlowering plants relies greatly on precise timing of thefloral transition,which isnely modulated by a complex network offloral regulators.As a mainfloral integrator,FLOWERING LOCUS T(FT)is also an essential constituent of theflorigen that is transported from leaves to shoot apices to induceflowering.FT is specically transcribed in leaf vascular tissues,where its production is suppressed by manyflowering repressors,including the MYB transcription factor EARLY FLOWERING MYB PROTEIN(EFM).Here,we show that a plant CTD phosphatase,C-TERMINAL DOMAIN PHOSPHATASE-LIKE 2(CPL2),suppresses FT expression in leaf vascular tissues by modulating the binding activity of EFM.CPL2 interacts with and dephosphorylates EFM to facilitate the binding of dephosphorylated EFM to FT chromatin,thereby inhibitingflowering.Our results suggest that CPL2-mediated dephosphorylation of thefloral repressor EFM serves as a molecular switch,adding another layer of regulation tone-tune FT transcription and ensure thatflowering occurs at an appropriate time.

关 键 词：CTD phosphatase MYB protein FLORIGEN FT FLOWERING ARABIDOPSIS 

分 类 号：Q94[生物学—植物学]