SP-141 targets Trs85 to inhibit rice blast fungus infection and functions as a potential broadspectrum antifungal agent  

作　　者：Xi-Yu Wu Bo Dong Xue-Ming Zhu Ying-Ying Cai Lin Li Jian-Ping Lu Bin Yu Jing-Li Cheng Fei Xu Jian-Dong Bao Ying Wang Xiao-Hong Liu Fu-Cheng Lin 

机构地区：[1]State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Institute of Biotechnology,Zhejiang University,Hangzhou 310058,Zhejiang Province,China [2]State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310058,Zhejiang Province,China [3]Department of Pharmacology and Nutritional Science,College of Medicine,The University of Kentucky,Lexington,KY 40506,USA [4]Markey Cancer Center,College of Medicine,The University of Kentucky,Lexington,KY 40506,USA [5]College of Life Sciences,Zhejiang University,Hangzhou 310058,Zhejiang Province,China [6]Institute of Pesticide and Environmental Toxicology,Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects,Zhejiang University,Hangzhou 310058,Zhejiang Province,China [7]Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201106,Shanghai,China

出　　处：《Plant Communications》2024年第2期169-184,共16页植物通讯（英文）

基　　金：supported by the Key Research and Development Project of Zhejiang Province,China (2021C02010);the National Natural Science Foundation of China (32270201,31972216,and 31970140);the Special Project for the Selection and Breeding of New Agricultural Varieties in Zhejiang Province,China (2021C02064);supported by a grant,Organism Interaction,from Zhejiang Xianghu Laboratory (to F.-C.L.).

摘　　要：Rice blast is a devastating disease worldwide,threatening rice production and food security.The blast fun-gus Magnaporthe oryzae invades the host via the appressorium,a specialized pressure-generating struc-ture that generates enormous turgor pressure to penetrate the host cuticle.However,owing to ongoing evolution of fungicide resistance,it is vitally important to identify new targets and fungicides.Here,we show that Trs85,a subunit of the transport protein particle III complex,is essential for appressorium-mediated infection in M.oryzae.We explain how Trs85 regulates autophagy through Ypt1(a small guano-sine triphosphatase protein)in M.oryzae.We then identify a key conserved amphipathic a helix within Trs85 that is associated with pathogenicity of M.oryzae.Through computer-aided screening,we identify a lead compound,SP-141,that affects autophagy and the Trs85–Ypt1 interaction.SP-141 demonstrates a substantial capacity to effectively inhibit infection caused by the rice blast fungus while also exhibiting wide-ranging potential as an antifungal agent with broad-spectrum activity.Taken together,our data show that Trs85 is a potential new target and that SP-141 has potential for the control of rice blast.Ourfindings thus provide a novel strategy that may help in thefight against rice blast.

关 键 词：Magnaporthe oryzae rice blast TRAPPIII complex trs85 FUNGICIDE 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]