Two haplotype-resolved genome assemblies for AAB allotriploid bananas provide insights into banana subgenome asymmetric evolution and Fusarium wilt control  

作　　者：Wen-Zhao Xie Yu-Yu Zheng Weidi He Fangcheng Bi Yaoyao Li Tongxin Dou Run Zhou Yi-Xiong Guo Guiming Deng Wenhui Zhang Min-Hui Yuan Pablo Sanz-Jimenez Xi-Tong Zhu Xin-Dong Xu Zu-Wen Zhou Zhi-Wei Zhou Jia-Wu Feng Siwen Liu Chunyu Li Qiaosong Yang Chunhua Hu Huijun Gao Tao Dong Jiangbo Dang Qigao Guo Wenguo Cai Jianwei Zhang Ganjun Yi Jia-Ming Song Ou Sheng Ling-Ling Chen 

机构地区：[1]Institute of Fruit Tree Research,Guangdong Academy of Agricultural Sciences,Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization,Ministry of Agriculture and Rural Affairs,Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research,Guangzhou 510640,China [2]College of Informatics,Huazhong Agricultural University,Wuhan 430070,China [3]State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,College of Life Science and Technology,Guangxi University,Nanning 530004,China [4]College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China

出　　处：《Plant Communications》2024年第2期243-260,共18页植物通讯（英文）

基　　金：funded by the Strategy of Rural Vitalization of Guangdong Provinces (2022-NPY-00-003,2022-NJS-00-001);the National Natural Science Foundation of China (32270712);the earmarked fund for CARS (CARS-31-01);GDAAS (202102TD,R2020PY-JX002);the Ba-Gui Scholar Program of Guangxi (to Z.-G.H);the Laboratory of Lingnan Modern Agriculture Project (NT2021004);the Maoming Branch Grant (2021TDQD003).

摘　　要：Bananas(Musa spp.)are one of the world’s most important fruit crops and play a vital role in food security for many developing countries.Most banana cultivars are triploids derived from inter-and intraspecific hybrid-izations between the wild diploid ancestor species Musa acuminate(AA)and M.balbisiana(BB).We report two haplotype-resolved genome assemblies of the representative AAB-cultivated types,Plantain and Silk,and precisely characterize ancestral contributions by examining ancestry mosaics across the genome.Widespread asymmetric evolution is observed in their subgenomes,which can be linked to frequent homol-ogous exchange events.We reveal the genetic makeup of triploid banana cultivars and verify that subge-nome B is a rich source of disease resistance genes.Only 58.5%and 59.4%of Plantain and Silk genes,respectively,are present in all three haplotypes,with>50%of genes being differentially expressed alleles in different subgenomes.We observed that the number of upregulated genes in Plantain is significantly higher than that in Silk at one-week post-inoculation with Fusarium wilt tropical race 4(Foc TR4),which con-firms that Plantain can initiate defense responses faster than Silk.Additionally,we compared genomic and transcriptomic differences among the genes related to carotenoid synthesis and starch metabolism between Plantain and Silk.Our study provides resources for better understanding the genomic architecture of culti-vated bananas and has important implications for Musa genetics and breeding.

关 键 词：bananas PLANTAIN Silk asymmetric evolution homologous exchange Fusarium wilt 

分 类 号：S668.1[农业科学—果树学]