Human extracellular matrix(ECM)-like collagen and its bioactivity  

作　　者：Hui Zhou Wenwei Li Lixin Pan Tianci Zhu Teng Zhou E.Xiao Qiang Wei 

机构地区：[1]State Key Laboratory of Polymer Materials and Engineering,College of Polymer Science and Engineering,Sichuan University,Chengdu 610065,China [2]Hunan Maybio Bio-Pharmaceutical Co.,Ltd,Changsha 410000,China

出　　处：《Regenerative Biomaterials》2024年第4期14-26,共13页再生生物材料（英文版）

基　　金：the National Key R&D Program of China(2021YFB3800705);the Science and Technology Innovation Program of Hunan Province(2022RC4013);the Science and Technology Program of Changsha(kq2303005).

摘　　要：Collagen,the most abundant structural protein in the human extracellular matrix(ECM),provides essential support for tissues and guides tissue development.Despite its widespread use in tissue engineering,there remains uncertainty regarding the optimal selection of collagen sources.Animal-derived sources pose challenges such as immunogenicity,while the recombinant system is hindered by diminished bioactivity.In this study,we hypothesized that human ECM-like collagen(hCol)could offer an alternative for tissue engineering.In this study,a facile platform was provided for generating hCol derived from mesenchymal stem cells with a hierarchical structure and biochemical properties resembling native collagen.Our results further demonstrated that hCol could facilitate basal biological behaviors of human adipose-derived stem cells,including viability,proliferation,migration and adipocyte-like phenotype.Additionally,it could promote cutaneous wound closure.Due to its high similarity to native collagen and good bioactivity,hCol holds promise as a prospective candidate for in vitro and in vivo applications in tissue engineering.

关 键 词：extracellular matrix native-like collagen cell-derived matrix hierarchical structure 

分 类 号：R318[医药卫生—生物医学工程]