Methacrylated gelatin and platelet-rich plasma based hydrogels promote regeneration of critical-sized bone defects  

作　　者：Shichao Lian Zhiyu Mu Zhengchao Yuan Muhammad Shafiq Xiumei Mo Weidong Mu 

机构地区：[1]Department of Traumatic Orthopaedics,Shandong Provincial Hospital,Shandong University,Jinan,Shandong 250012,China [2]Zoucheng People’s Hospital,Zoucheng,Shandong 273500,China [3]Department of Medical Physics and Biomedical Engineering,University of London,London WC1E 6BT,UK [4]State Key Laboratory for Modification of Chemical Fibers and Polymer Materials,Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine,College of Biological Science and Medical Engineering,Donghua University,Shanghai 201620,PR China [5]Innovation Center of NanoMedicine(iCONM),Kawasaki Institute of Industrial Promotion,Kawasaki-Ku,Kawasaki 210-0821,Japan

出　　处：《Regenerative Biomaterials》2024年第4期66-81,共16页再生生物材料（英文版）

基　　金：funded by Donghua University Postgraduate Innovation and Entrepreneurship Ability Training Program(yjssc2023002);supported by Science and Technology Commission of Shanghai Municipality,China(grant numbers 20S31900900 and 20DZ2254900);Sino German Science Foundation Research Exchange Center,China(M-0263);China Education Association for International Exchange(2022181).

摘　　要：Physiological repair of large-sized bone defects requires instructive scaffolds with appropriate mechanical properties,biocompatibility,biodegradability,vasculogenic ability and osteo-inductivity.The objective of this study was to fabricate in situ injectable hydrogels using platelet-rich plasma(PRP)-loaded gelatin methacrylate(GM)and employ them for the regeneration of large-sized bone defects.We performed various biological assays as well as assessed the mechanical properties of GM@PRP hydrogels alongside evaluating the release kinetics of growth factors(GFs)from hydrogels.The GM@PRP hydrogels manifested sufficient mechanical properties to support the filling of the tissue defects.For biofunction assay,the GM@PRP hydrogels significantly improved cell migration and angiogenesis.Especially,transcriptome RNA sequencing of human umbilical vein endothelial cells and bone marrow-derived stem cells were performed to delineate vascularization and biomineralization abilities of GM@PRP hydrogels.The GM@PRP hydrogels were subcutaneously implanted in rats for up to 4 weeks for preliminary biocompatibility followed by their transplantation into a tibial defect model for up to 8 weeks in rats.Tibial defects treated with GM@PRP hydrogels manifested significant bone regeneration as well as angiogenesis,biomineralization,and collagen deposition.Based on the biocompatibility and biological function of GM@PRP hydrogels,a new strategy is provided for the regenerative repair of large-size bone defects.

关 键 词：large-sized bone defect repair platelet-rich plasma HYDROGEL tissue scaffold gelatin methacrylate 

分 类 号：O63[理学—高分子化学]