氧化石墨烯对树莓组培苗玻璃化影响  

Alleviation of Vitrification in Raspberry Tissue Culture Seedlings by Graphene Oxide

作　　者：黄忠越 HUANG Zhong-Yue(Sangganhe Poplar High-yield Forest Bureau,Datong,Shanxi 037000)

机构地区：[1]山西省桑干河杨树丰产林实验局,山西大同037000

出　　处：《陕西林业科技》2024年第5期7-12,17,共7页Shaanxi Forest Science and Technology

基　　金：2018年山西省科技成果转化引导专项项目(201804D131041)。

摘　　要：为了解氧化石墨烯(Grapherie oxide)对植物组培苗玻璃化的影响,本文将不同浓度氧化石墨烯应用于树莓组培苗初代培养和增殖培养阶段。结果表明,4 mg·L^(-1)氧化石墨烯、10 mg·L^(-1)国光·安盖、15 mg·L^(-1)国光·氨基酸铁,能有效降低树莓组培苗初代培养阶段玻璃化率,提升苗木的萌芽率和苗高;2 mg·L^(-1)氧化石墨烯、5 mg·L^(-1)国光·安盖、10 mg·L^(-1)国光·氨基酸铁,能有效降低树莓组培苗增殖培养阶段的玻璃化率,提升苗木的增殖系数和生长量。研究认为,通过将适宜浓度的氧化石墨烯、国光·安盖、国光·氨基酸铁加入植物组织培养初代和增殖培养基中,能够有效降低组培苗玻璃化率和提高组培苗萌芽率、增殖系数。In order to understand the effect of graphene oxide on the vitrification of plant tissue cultured seedlings,different concentrations of graphene oxide to the initial and proliferation stages of Rubus corchorifolius tissue cultured seedlings were applied.The result showed that 4 mg·L^(-1)graphene oxide,10 mg·L^(-1)Guoguang·Angai,and 15 mg·L^(-1)Guoguang·Iron Amino Acid could effectively reduce the vitrification rate of raspberry tissue culture seedlings during the initial culture stage and improve their germination rate and seedling height.Meanwhile,2 mg·L^(-1)graphene oxide,5 mg·L^(-1)Guoguang·Angai,and 10 mg·L^(-1)Guoguang·Amino Acid Iron could effectively reduce the vitrification rate of raspberry tissue culture seedlings during the proliferation culture stage and enhance their proliferation coefficient and growth.The study concluded that adding appropriate concentrations of graphene oxide,Guoguang·Angai,and Guoguang·Iron Amino Acid to the initial and proliferation media for plant tissue culture could effectively reduce the vitrification rate and improve the germination rate and proliferation coefficient of tissue culture seedlings.

关 键 词：树莓 组培苗 玻璃化 氧化石墨烯 含氨基酸水溶肥料 

分 类 号：S663.2[农业科学—果树学]