废弃烟梗制备细菌纤维素的最佳条件及其吸附性能研究  

Study on the optimal conditions and adsorption performance of bacterial cellulose prepared from discarded tobacco stems

作　　者：周猛 王龙飞 何爱民 陈伟华 王晓如 沈薇 来苗 崔冰 肖梦兰 赵铭钦[1] ZHOU Meng;WANG Longfei;HE Aimin;CHEN Weihua;WANG Xiaoru;SHEN Wei;LAI Miao;CUI Bing;XIAO Menglan;ZHAO Mingqin(College of Tobacco Science,Flavors and Fragrance Enginering&Technology Research Center of Henan Province,Henan Agricultural University,Zhengzhou 450046,China;Technology Center,China Tobacco Hebei Industrial Co.,Ltd.,Shijiazhuang 050000,China)

机构地区：[1]河南农业大学烟草学院,河南省香精香料与调香工程技术研究中心,河南郑州450046 [2]河北中烟工业有限责任公司技术中心,河北石家庄050000

出　　处：《河南农业大学学报》2024年第5期746-756,共11页Journal of Henan Agricultural University

基　　金：河南省自然科学基金项目(232300421257);河北中烟工业有限责任公司重点科技项目(20231300003400038)。

摘　　要：【目的】探究以废弃烟梗源制备细菌纤维素的最佳条件及所制备样品的吸附性能。【方法】使用3个烟草产地(河南、陕西、湖南)的废弃烟梗水提物作为发酵培养基,制备了3种细菌纤维素(bacterial cellulose,BC),以BC产量为指标,对种龄、接种量、初始pH值、装液量、温度、时间6个发酵条件进行了优化,对不同的废弃烟梗水提物培养基和常规培养基所得BC的结构进行表征,并对重金属离子的吸附性能进行了对比。【结果】1)在不添加额外营养物质的情况下,烟梗水提物可直接作为发酵培养基生产细菌纤维素。2)发酵制备细菌纤维素的最佳条件分别为:种龄18 h,接种量10%,初始pH值5.5,装液量50 mL,温度30℃,时间9~11 d。3)细菌纤维素产量与烟梗水提物中糖含量呈正相关关系。4)烟梗水提物制备的BC通过扫描电子显微镜(scanning electron microscopy,SEM)、傅里叶变换红外光谱(fourier transform infrared spectroscopy,FTIR)、X射线衍射(X-ray diffraction,XRD)表征发现,其都具有BC典型的精细三维纤维网状结构,纤维直径约为30~100 nm,无明显差异且SX-BC结晶度较高。5)4种BC均能吸附溶液中的Cu^(2+)和Zn^(2+),其中SX-BC对Cu^(2+)和Zn^(2+)的吸附效果最佳,分别达到36和39 mg·g^(-1)。【结论】SX-BC的发酵产量最高,对Cu^(2+)和Zn^(2+)的吸附效果最好。【Objective】To explore the optimal preparation conditions for bacterial cellulose derived from discarded tobacco stems and the adsorption performances of the prepared samples.【Method】Three types of bacterial cellulose(BC)were prepared using discarded tobacco stem water extracted from three production areas(Henan,Shaanxi,and Hunan)as fermentation media.The BC yield was used as an indicator to optimize six fermentation conditions:seed age,inoculation amount,initial pH value,triangular bottle liquid volume,temperature,and time.The structure of BC obtained from different discarded tobacco stem water extract media and conventional media was characterized,and the adsorption performances of heavy metal ions were compared.【Result】1)Without adding additional nutrients,tobacco stem water extract can be directly used as a fermentation medium to produce bacterial cellulose.2)The optimal conditions for fermenting bacterial cellulose are as follows:seed age of 18 hours,inoculation amount of 10%,initial pH value 5.5,liquid volume of 50 mL,temperature of 30℃,and time of 9-11 days.3)There is a positive correlation between bacterial cellulose production and sugar content in tobacco stem water extracts.4)The BC prepared from tobacco stem water extract was characterized by scanning electron microscopy(SEM),fourier transform infrared spectroscopy(FTIR),and X-ray diffraction(XRD),and it was found that it has a typical fine three-dimensional fiber network structure of BC.The fiber diameter is about 30-100 nm,with no significant difference,and the SX-BC crystallinity is relatively high.5)All four types of BC can adsorb Cu^(2+)and Zn^(2+)in solution,among which SX-BC has the best adsorption effect on Cu^(2+)and Zn^(2+),reaching 36 mg·g^(-1)and 39 mg·g^(-1),respectively.【Conclusion】The SX-BC has the highest fermentation yield and the best adsorption effect on Cu^(2+)and Zn^(2+).

关 键 词：烟梗 细菌纤维素 吸附 形貌表征 

分 类 号：X795[环境科学与工程—环境工程]