不同浓度外源硒对中等盐胁迫下小麦幼苗的影响  

Effects of different concentrations of exogenous selenium on wheat seedlings under moderate salt stress

作　　者：王浩权 杨东霞 刘红恩[1,2] 刘亥扬 刘文艺 陶志康 王灵璐 聂兆君 WANG Haoquan;YANG Dongxia;LIU Hong’en;LIU Haiyang;LIU Wenyi;TAO Zhikang;WANG Linglu;NIE Zhaojun(College of Resources and Environment,Henan Agricultural University,Zhengzhou 450046,China;Soil Pollution Control Engineering Technology Research Center of Henan Province,Zhengzhou 450046,China;Product Quality Inspection and Testing Center of Administration for Market Regulation of Luoyang City,Luoyang 471032,China)

机构地区：[1]河南农业大学资源与环境学院,河南郑州450046 [2]河南省土壤污染控制工程技术研究中心,河南郑州450046 [3]洛阳市市场监督管理局产品质量检验检测中心,河南洛阳471032

出　　处：《河南农业大学学报》2024年第5期791-799,共9页Journal of Henan Agricultural University

基　　金：“十四五”国家重点研发计划项目(2021YFD1700900);河南省科技攻关项目(232102320117);河南省高等学校重点科研项目(22A210018)。

摘　　要：【目的】探究不同浓度外源硒(Se)对小麦幼苗中等盐胁迫(100 mmol·L^(-1))的缓解效果。【方法】采用水培试验,以小麦百农207和亚硒酸钠为供试材料,设置CK(不添加NaCl和Se)、Na(100 mmol·L^(-1)NaCl)、NaSe5(100 mmol·L^(-1)NaCl^(+)5μmol·L^(-1)Se)、NaSe10(100 mmol·L^(-1)NaCl^(+)10μmol·L^(-1)Se)、NaSe20(100 mmol·L^(-1)NaCl^(+)20μmol·L^(-1)Se)5个处理,测定小麦幼苗生物量、根系参数、光合特性(光合色素含量和光合参数)及离子平衡(Na^(+)、K^(+)含量及其转运系数)。【结果】与CK相比,中等盐胁迫显著降低了小麦幼苗干物质积累量、根系形态参数、光合特性及小麦幼苗地上部和根部的K^(+)含量和K^(+)转运系数,但是显著增加了小麦Na^(+)含量和Na^(+)转运系数;在中等盐胁迫下,不同浓度外源Se对小麦幼苗影响不同,其中,施用5μmol·L^(-1)Se能够最大程度增加小麦幼苗的干质量、总根表面积、叶绿素a和净光合速率,分别增加了15.00%、44.36%、34.65%和39.21%,同时降低小麦幼苗Na^(+)转运系数、地上部和根部Na^(+)/K^(+)比值,分别降低了11.13%、42.37%和51.18%。【结论】适宜浓度的外源Se(5μmol·L^(-1))能够有效缓解中等盐胁迫对小麦幼苗生长发育带来的损害。【Objective】To explore the alleviating effects of different concentrations of exogenous selenium(Se)on moderate salt stress(100 mmol·L^(-1))of wheat seedlings.【Method】A hydroponic experiment was carried out with wheat Bainong 207 and sodium selenite as test materials.Five treatments were set up:CK(without adding NaCl and Se),Na(100 mmol·L^(-1) NaCl),NaSe5(100 mmol·L^(-1) NaCl^(+)5μmol·L^(-1) Se),NaSe10(100 mmol·L^(-1) NaCl^(+)10μmol·L^(-1) Se)and NaSe20(100 mmol·L^(-1) NaCl^(+)20μmol·L^(-1) Se).Wheat seedling biomass,root parameters,photosynthetic characteristics(photosynthetic pigment content and photosynthetic parameters)and ion balance(Na^(+),K^(+)content and their transport coefficients)were determined.【Result】Compared with CK,the 100 mmol·L^(-1) NaCl stress significantly reduced dry matter accumulation,root morphological parameters,photosynthetic characteristics,K^(+)content and K^(+)transport coefficient in shoot and root,but significantly increased Na^(+)content and Na^(+)transport coefficient in roots and shoot of wheat seedlings.Under medium salt stress,wheat seedling exhibited varying responses to different concentrations of exogenous Se.Among them,the application of 5μmol·L^(-1) Se could increase dry weight,total root surface area,chlorophyll a and net photosynthetic rate of wheat seedlings to the greatest extent by 15.00%,44.36%,34.65%and 39.21%,respectively.At the same time,this treatment decreased the Na^(+)transport coefficient and the Na^(+)/K^(+)ratio in the ground and root of wheat seedlings to 11.13%,42.37%and 51.18%.【Conclusion】Appropriate concentration of exogenous Se(5μmol·L^(-1))can effectively alleviate the damage of moderate salt stress on the growth and development of wheat seedlings.

关 键 词：小麦 中等盐胁迫 外源硒 光合特性 离子平衡 

分 类 号：S512.1[农业科学—作物学] S143.7