Design-build-test of recombinant Bacillus subtilis chassis cell by lifespan engineering for robust bioprocesses  

作　　者：Kexin Ren Qiang Wang Jianghua Chen Hengwei Zhang Zhoule Guo Meijuan Xu Zhiming Rao Xian Zhang 

机构地区：[1]Key Laboratory of Industrial Biotechnology of the Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi,Jiangsu,214122,China [2]Yixing Institute of Food and Biotechnology Co.,Ltd,Yixing,214200,China

出　　处：《Synthetic and Systems Biotechnology》2024年第3期470-480,共11页合成和系统生物技术（英文）

基　　金：National Key Research and Development Program of China(No.2020YFA0908300);National Natural Science Foundation of China(No.32171471);Postgraduate Research&Practice Innovation Program of Jiangsu Province(SJCX23_1241),the Fundamental Research Funds for the Central Universities,The Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions,Top-notch Academic Programs Project of Jiangsu Higher Education Institutions.

摘　　要：Microbial cell factories utilize renewable raw materials for industrial chemical production,providing a promising path for sustainable development.Bacillus subtilis is widely used in industry for its food safety properties,but challenges remain in the limitations of microbial fermentation.This study proposes a novel strategy based on lifespan engineering to design robust B.subtilis chassis cells to supplement traditional metabolic modification strategies that can alleviate cell autolysis,tolerate toxic substrates,and get a higher mass transfer efficiency.The modified chassis cells could produce high levels of L-glutaminase,and tolerate hydroquinone to produceα-arbutin efficiently.In a 5 L bioreactor,the L-glutaminase enzyme activity of the final strain CRE15TG was increased to 2817.4±21.7 U m L^(-1),about 1.98-fold compared with that of the wild type.Theα-arbutin yield of strain CRE15A was increased to 134.7 g L^(-1),about 1.34-fold compared with that of the WT.To our knowledge,both of the products in this study performed the highest yields reported so far.The chassis modification strategy described in this study can Improve the utilization efficiency of chassis cells,mitigate the possible adverse effects caused by excessive metabolic modification of engineered strains,and provide a new idea for the future design of microbial cell factories.

关 键 词：Bacillus subtilis Chassis cell Lifespan engineering Robustness Industrial production 

分 类 号：Q93[生物学—微生物学]