miR-194和miR-155与乙型病毒性肝炎肝硬化患者乙型肝炎病毒DNA及预后的关系  

Relationship between miR-194 and miR-155 and hepatitis B virus DNA and prognosis in patients with viral hepatitis B cirrhosis

作　　者：刘鹏[1] 王辉[2] 闫国贝 LIU Peng;WANG Hui;YAN Guo-bei(Department of Clinical Laboratory,the Second People's Hospital of Jiaozuo,Jiaozuo,Henan 454001,China;Department of Medical Records Management,the Second People's Hospital of Jiaozuo,Jiaozuo,Henan 454001,China)

机构地区：[1]焦作市第二人民医院检验科,河南焦作454001 [2]焦作市第二人民医院病案管理科,河南焦作454001

出　　处：《医药论坛杂志》2024年第16期1716-1720,1726,共6页Journal of Medical Forum

摘　　要：目的分析miR-194和miR-155与乙型病毒性肝炎肝硬化患者乙型肝炎病毒(hepatitis B virus,HBV)DNA载量之间的关系,并探究miR-194、miR-155及HBV DNA载量对患者预后不良的预测价值。方法选择2020年6月至2023年6月焦作市第二人民医院收治的未给予抗病毒治疗的HBV感染患者200例作为调查对象,并根据其疾病类型划分为HBV携带组(n=59)、慢性乙型肝炎(chronic hepatitis B,CHB)组(n=77)和肝硬化组(n=64),对肝硬化患者随访3个月并统计其预后情况,比较三组患者外周血miR-194、miR-155表达量及血清HBV DNA载量,采用Pearson相关检验分析肝硬化患者外周血miR-194、miR-155表达量与血清HBV DNA载量的相关性,同时比较不同预后肝硬化患者外周血miR-194、miR-155表达量及血清HBV DNA载量,绘制受试者工作特征(receiver operating characteristic,ROC)曲线及计算曲线下面积(area under curve,AUC)分析外周血miR-194、miR-155表达量及血清HBV DNA载量对肝硬化患者预后不良的预测价值。结果肝硬化组和CHB组患者外周血miR-194、miR-155表达量及血清HBV DNA载量显著高于HBV携带组(P<0.05),且肝硬化组显著高于CHB组(P<0.05);Pearson相关检验结果显示,外周血miR-194、miR-155表达量与血清HBV DNA载量均呈显著正相关(P<0.05);预后不良组患者外周血miR-194、miR-155表达量及血清HBV DNA载量显著高于预后良好组(P<0.05);外周血miR-194、miR-155表达量、血清HBV DNA载量及三者联合预测肝硬化患者预后不良的AUC分别为0.754、0.759、0.801和0.895。结论乙型病毒性肝炎肝硬化患者外周血miR-194、miR-155表达量显著上升,并与乙型病毒性肝炎肝硬化患者HBV DNA载量及预后密切相关,同时miR-194、miR-155联合HBV DNA载量对乙型病毒性肝炎肝硬化患者预后不良具有一定的预测价值,可为临床提供参考依据。Objective To analyse the relationship between miR-194 and miR-155 and hepatitis B virus(HBV)DNA load in cirrhotic patients with viral hepatitis B,and to investigate the predictive value of miR-194,miR-155 and HBV DNA load in patients with poor prognosis.Methods Totally 200 patients with HBV infection who were not treated with antiviral therapy in the Second People's Hospital of Jiaozuo from June 2020 to June 2023 were selected as the subjects of investigation.According to the disease types,they were divided into HBV carrier group(n=59),chronic hepatitis B(CHB)group(n=77)and cirrhosis group(n=64).The patients with cirrhosis were followed up for 3 months and the prognosis was statistically analyzed.The expression levels of peripheral blood miR-194 and miR-155 and serum HBV DNA load were compared among the three groups of patients.Pearson correlation test was used to analyze the correlation between expression levels of miR-194 and miR-155 in peripheral blood and serum HBV DNA load in patients with cirrhosis.The expression levels of peripheral blood miR-194 and miR-155 and serum HBV DNA load in patients with cirrhosis with different prognosis status were compared.Receiver operating characteristic(ROC)curve was drawn and the area under the curve(AUC)was calculated to analyze the predictive value of peripheral blood miR-194 and miR-155 and serum HBV DNA load on the poor prognosis of patients with cirrhosis.Results The expression levels of miR-194 and miR-155 in peripheral blood and serum HBV DNA load in cirrhosis group and CHB group were significantly higher than those in HBV carrier group(P<0.05),and the above indicators in cirrhosis group were significantly higher than those in CHB group(P<0.05).Pearson correlation test results showed that the expression levels of peripheral blood miR-194 and miR-155 were significantly positively correlated with serum HBV DNA load(P<0.05).The expression levels of peripheral blood miR 194 and miR-155 and serum HBV DNA load in poor prognosis group were significantly higher than those i

关 键 词：miR-194 MIR-155 乙型肝炎病毒 肝硬化 HBV DNA 预后 

分 类 号：R512.6[医药卫生—内科学]