Studies of polyol production by the yeast Yarrowia lipolytica growing on crude glycerol under stressful conditions  

作　　者：Eleni-Stavroula Vastaroucha Nikolaos G.Stoforos George Aggelis Seraphim Papanikolaou 

机构地区：[1]Department of Food Science and Human Nutrition,Agricultural University of Athens,75 Iera Odos,11855,Athens,Greece [2]Unit of Microbiology,Department of Biology,University of Patras,26504 Rio Patras,Greece

出　　处：《Carbon Resources Conversion》2024年第3期17-31,F0004,共16页碳资源转化（英文）

基　　金：supported by the project entitled“Adding value to biodiesel-derived crude glycerol with the use of Chemical and Microbial Technology”(Acronym:Addvalue2glycerol,project codeΤ1ЕΔΚ-03002)financed by the Ministry of National Edu-cation and Religious Affairs,Greece(project action:“Investigate-Create-Innovate 2014-2020,Intervention II”)。

摘　　要：Crude glycerol,the principal by-product of biodiesel production process,was employed as substrate by three wild-type Yarrowia lipolytica strains(ACA-YC 5030,LMBF 20 and NRRL Y-323).Stressful conditions(low pH value=2.0±0.3,low incubation temperature T=20±1℃,non-aseptic conditions)were employed.Inter-esting production of yeast biomass and polyols(viz.erythritol,mannitol and arabitol)was noted at pH=2.0±0.3 and T=20±1℃.Strains failed to produce significant quantities of cellular lipid,while variable quantities of intra-cellular polysaccharides were produced.Fermentations under previously pasteurized media supported significant biomass and polyols production for most of the tested strains,while only one strain(NRRL Y-323),managed to produce polyols at media that were not previously thermally treated at all.The production of mannitol was favored at low initial glycerol(Glol0)concentrations,whereas higher Glol0 quantities favored the biosynthesis of erythritol.For the strain NRRL Y-323,highly aerated/agitated bioreactor trials showed different physiological profiles as compared to the respective flask experiments.Finally,in flask experiments with the strain NRRL Y-323 at high Glol0 amounts(≈140 g/L)at low medium pH(=2.0±0.3),a significant production of polyols(=84.2 g/L)with the corresponding remarkable conversion yield on glycerol consumed=62%w/w was achieved.Practical application:Renewable and biodegradable fuels,such as biodiesel,are safer and environmentally friendlier than the conventional petroleum diesel.Glycerol is a cost-effective substrate obtained as the main side-product from biodiesel production process and is currently being employed in the realm of Industrial Microbi-ology and Biotechnology to produce metabolic products with added value.Current research focuses on using glycerol as a starting substrate for biotechnological conversions aiming at producing,amongst other compounds,polyols,microbial biomass,citric acid,etc.from selected strains of the Generally RecognizedАs Safe(GRAS)yeast Yarro

关 键 词：Biomass GLYCEROL POLYOLS Yarrowia lipolytica 

分 类 号：TQ64[化学工程—精细化工]