Stimulated Raman scattering microscopy with phase-controlled light focusing and aberration correction for rapid and label-free, volumetric deep tissue imaging  

作　　者：Weiqi Wang Zhiwei Huang 

机构地区：[1]Optical Bioimaging Laboratory,Department of Biomedical Engineering,College of Design and Engineering,National University of Singapore,Singapore 117576

出　　处：《Opto-Electronic Advances》2024年第9期40-52,共13页光电进展（英文）

基　　金：supported by the Academic Research Fund(AcRF)from the Ministry of Education(MOE)(Tier 2(A-8000117-01-00);Tier 1(R397-000-334-114,R397-000-371-114,and R397-000-378-114);2024 Tsinghua-NUS Joint Research Initiative Fund,and the National Medical Research Council(NMRC)(A-0009502-01-00,and A-8001143-00-00),Singapore.

摘　　要：We report a novel stimulated Raman scattering(SRS)microscopy technique featuring phase-controlled light focusing and aberration corrections for rapid,deep tissue 3D chemical imaging with subcellular resolution.To accomplish phasecontrolled SRS(PC-SRS),we utilize a single spatial light modulator to electronically tune the axial positioning of both the shortened-length Bessel pump and the focused Gaussian Stokes beams,enabling z-scanning-free optical sectioning in the sample.By incorporating Zernike polynomials into the phase patterns,we simultaneously correct the system aberrations at two separate wavelengths(~240 nm difference),achieving a~3-fold enhancement in signal-to-noise ratio over the uncorrected imaging system.PC-SRS provides>2-fold improvement in imaging depth in various samples(e.g.,polystyrene bead phantoms,porcine brain tissue)as well as achieves SRS 3D imaging speed of~13 Hz per volume for real-time monitoring of Brownian motion of polymer beads in water,superior to conventional point-scanning SRS 3D imaging.We further utilize PC-SRS to observe the metabolic activities of the entire tumor liver in living zebrafish in cellsilent region,unraveling the upregulated metabolism in liver tumor compared to normal liver.This work shows that PCSRS provides unprecedented insights into morpho-chemistry,metabolic and dynamic functioning of live cells and tissue in real-time at the subcellular level.

关 键 词：SRS 3D imaging phase-controlled light focusing image aberration corrections deep tissue imaging 

分 类 号：TH742[机械工程—光学工程] Q-334[机械工程—仪器科学与技术]