LsMET1基因沉默对叶用莴苣高温抽薹的形态和表达分析  

Morphological and expression analysis of LsMET1 gene silencing on high temperature bolting of leaf lettuce

作　　者：谭青青 田玉凤 王月英[2] 刘婷婷 韩莹琰[1] 刘超杰[1] 王惠玉 范双喜 郝敬虹[1] TAN Qingqing;TIAN Yufeng;WANG Yueying;LIU Tingting;HAN Yingyan;LIU Chaojie;WANG Huiyu;FAN Shuangxi;HAO Jinghong(College of Plant Science and Technology/Beijing Key Laboratory of New Technology in Agricultural Application,Beijing University of Agriculture,Beijing 102206,China;Beijing Agricultural Vocational College,Beijing 102442,China)

机构地区：[1]北京农学院植物科学技术学院/农业应用新技术北京市重点试验室,北京102206 [2]北京农业职业学院,北京102442

出　　处：《北京农学院学报》2024年第4期38-43,共6页Journal of Beijing University of Agriculture

基　　金：北京市自然科学基金-教委联合重点资助项目(KZ20231002034);北京市种质创制和品种选育联合攻关项目(G20220628003);“生菜高产优质品种选育及配套栽培技术研究”(XY-TD-22-01)。

摘　　要：【目的】为了探究LsMET1基因在叶用莴苣高温抽薹过程中的功能,培育具有抗抽薹特性的叶用莴苣新品种提供理论依据。【方法】以易抽薹叶用莴苣品种‘GB-30’为材料,克隆了LsMET1基因,并利用生物信息学方法分析其序列特征,并通过病毒诱导基因沉默、荧光定量PCR等技术研究该基因在抽薹过程中的功能。【结果】LsMET1基因编码区序列(CDS)长度为993 bp,编码的蛋白质由330个氨基酸残基构成,相对分子量约为37 kDa,理论等电点(pI)为7.57;荧光定量PCR结果显示,在高温诱导的环境条件下,LsMET1表达显著下降。与野生型植株相比,LsMET1基因沉默植株的茎长明显缩短。【结论】LsMET1基因与叶用莴苣高温抽薹密切相关,可能促进叶用莴苣抽薹。[Objective]This study aimed to analyze the mechanism of the LsMET1 gene in the process of bolting induction of lettuce under high temperatures and to provide the theoretical basis for breeding new varieties of lettuce with bolting resistance in the future.[Methods]Using the lettuce variety‘GB-30’for easy bolting leaf as the material,the LsMET1 gene was cloned and its sequence characteristics were analyzed by bioinformatics methods and the function of the gene in the bolting process was studied through virus induced gene silencing,quantitative real time PCR and other technologies.[Results]The coding region sequence(CDS)of LsMET1 gene was 993 bp and the encoded protein was composed of 330 amino acid residues,with a molecular weight of about 37 kDa and a theoretical isoelectric point(pI)of 7.57.Quantitative real time PCR results showed that LsMET1 expression significantly decreased in high temperature.Compared with wild-type plants,the stem length of LsMET1 gene silencing plants was significantly shortened.[Conclusion]The LsMET1 might play a positive role in regulating the high-temperature-induced bolting of leaf lettuce.

关 键 词：叶用莴苣 LsMET1 抽薹 瞬时沉默 

分 类 号：S636.2[农业科学—蔬菜学]