铁甲秋海棠DNA甲基转移酶全基因组鉴定及表达分析  

Identification and Expression Analysis of DNA Methyltransferase in Begonia masoniana

作　　者：陈婷欣 符敏[3] 李娜[2] 杨蕾蕾[2] 李凌飞[2] 钟春梅 Tingxin Chen;Min Fu;Na Li;Leilei Yang;Lingfei Li;Chunmei Zhong(Guangdong Engineering Technology Research Center of Agricultural and Forestry Biomass,Key Laboratory of Energy Plants Resource and Utilization,Ministry of Agriculture and Rural Affairs,Institute of Biomass Engineering,South China Agricultural University,Guangzhou 510642,China;Key Laboratory of Southern Subtropical Plant Diversity,Fairy Lake Botanical Garden,Shenzhen&Chinese Academy of Sciences,Shenzhen 518004,China;Zhongshan Agricultural Science and Technology Extension Center,Zhongshan 528400,China)

机构地区：[1]华南农业大学生物质工程研究院,农业农村部能源植物资源与利用重点实验室,广东省农林生物质工程技术研究中心,广州510642 [2]深圳市中国科学院仙湖植物园,深圳市南亚热带植物多样性重点实验室,深圳518004 [3]中山市农业科技推广中心,中山528400

出　　处：《植物学报》2024年第5期726-737,共12页Chinese Bulletin of Botany

基　　金：广东省自然科学基金(No.2021A1515011315);深圳市城管科研项目(No.202408,No.202205)。

摘　　要：DNA甲基化是重要的表观遗传修饰之一,参与调控植物基因组稳定性、发育及胁迫响应等过程。DNA甲基转移酶是DNA甲基化的关键酶。为了解铁甲秋海棠(Begonia masoniana)DNA甲基转移酶的功能,采用生物信息学方法从铁甲秋海棠基因组中鉴定出5个编码DNA甲基转移酶的基因。根据序列特征将其分为CMT、MET和DRM三类。不同类别成员的基因序列长度和内含子数量存在明显差异,但同类成员的基因结构和保守结构域具有高度保守性。这些蛋白均定位于细胞核,且基因启动子含有大量的光响应、MYB结合及植物激素响应等元件。激素响应模式分析表明,CMT3类在GA、SA和NAA处理下基因表达显著降低,CMT2类在MeJA和NAA处理下基因表达显著降低,而MET类和DRM类分别在GA和ABA处理下基因表达显著升高。此外,组织特异性分析发现,叶片中BmaCMT2-5和BmaDRM2-2的表达量明显高于其它组织器官,且这2个酶的编码基因与BmaMET1-15在叶片红色部分的表达高于绿叶部分,推测这3个DNA甲基转移酶可能在叶斑形成过程中发挥重要作用。INTRODUCTION:DNA methylation is one of the important epigenetic modifications involved in the regulation of plant genome stability,development and stress responses.DNA methylation introduces methylation groups into DNA molecules,thereby altering the activity of DNA segments.DNA methylation is catalyzed by DNA methyltransferase,a process by which methyl groups formed from S-adenosyl-L-methionine are transferred via covalent links to specific locations in the DNA sequence to form N4-methylcytosine,5-methylcytosine,N6-methyladenine,or 7-methylguanine.However,there are few reports about the effects of DNA methyltransferase on leaf variegation formation and stress response of Begonia.RATIONALE:Studies have shown that DNA methylation is involved in regulating the formation of leaf color,flower color and leaf variegation,as well as responses to stresses and hormones.As an endemic species of Begonia,Begonia masoniana has unique and beautiful leaf markings,pink,dark green and light green in different developmental stages.It has high ornamental value and is an excellent foliage plant.Therefore,based on the genomic data,this study conducted genome-wide identification and expression pattern analysis of DNA methyltransferase genes,aiming to explore the genetic resources that regulate the formation of leaf variegation.RESULTS:To investigate whether DNA methyltransferase is involved in the regulation of leaf variegation formation and stress response in B.masoniana,bioinformatics analysis was used to identify the genes encoding DNA methyltransferase.Five genes were obtained from the genome of B.masoniana.According to the protein structural characteristics,their encoded proteins were divided into three categories including CMT,MET and DRM.The sequence length and intron number of these genes were significantly categorized into different subgroups,but their structure and conserved domains in the same subgroup were highly conserved.In addition,all the encoded proteins were predicted to locate in the nucleus.The promoters of these ge

关 键 词：铁甲秋海棠 叶斑 DNA甲基转移酶 植物激素 

分 类 号：S682.19[农业科学—观赏园艺] Q943[农业科学—园艺学]