基于荧光寿命的人体正常和癌变结直肠组织分类识别  

Classification of Human Normal and Carcinoma Colorectal Tissue Based on Fluorescence Lifetime

作　　者：刘秉扬 林程[2] 颜慧贤 Liu Bingyang;Lin Cheng;Yan Huixian(College of Photonic&Electronic Engineering,Fujian Normal University,Fuzhou 350007,Fujian,China;?School of Mechanical and Electrical Engineering,Sanming University,Sanming 365004,Fujian,China)

机构地区：[1]福建师范大学光电与信息工程学院,福建福州350007 [2]三明学院机电工程学院,福建三明365004

出　　处：《应用激光》2024年第7期218-226,共9页Applied Laser

基　　金：福建省自然科学基金面上项目(2021J011130);福建省中青年教师教育科研项目(JAT190718);三明学院自然科学基金项目(KD22010P)。

摘　　要：基于瞬态自体荧光光谱技术,测量了人体正常和癌变结直肠组织在5个典型的最佳激发发射波长下的荧光寿命,比较评估了它们分类识别正常和癌变结直肠组织的性能。实验结果表明:正常和癌变结直肠组织位于340~380 nm、340~470 nm、405~515 nm和405~635 nm峰位的荧光寿命存在显著差异,通过约登指数分析得到其分类阈值分别为6.153 ns、5.847 ns、5.130 ns和6.414 ns;通过ROC曲线分析得到,在405~635 nm峰位可以获得最优的诊断性能,其灵敏度、特异性和AUC分别为85.7%、100%和0.908(P<0.001,95%CI:0.78~1.00)。该研究评估了自体荧光寿命可以作为一种有效的结直肠癌诊断指标,有望为结直肠癌的早期诊断提供参考价值。This study utilized transient autofluorescence spectroscopy to measure the autofluorescence lifetimes of normal and cancerous colorectal tissues at five optimal excitation-emission wavelengths. The performance of these lifetimes for classifying normal and cancerous tissues was evaluated. The results indicate significant differences in autofluorescence lifetimes between normal and cancerous colorectal tissues at wavelengths of 340~380 nm, 340~470 nm, 405~515 nm, and 405~635 nm. Jouden index analysis determined classification thresholds of 6.153 ns, 5.847 ns, 5.130 ns, and 6.414 ns, respectively. The receiver operating characteristic(ROC) curve analysis indicated that the peak diagnostic performance was achieved at 405~635 nm, with sensitivity, specificity, and area under the curve(AUC) values of 85.7%, 100%, and 0.908(P<0.001, 95% CI: 0.78~1.00), respectively. The findings suggest that autofluorescence lifetime measurements serve as a promising diagnostic biomarker for colorectal cancer, potentially aiding in its early detection.

关 键 词：结直肠癌 自体荧光光谱 内源性荧光团 荧光寿命 

分 类 号：O433.2[机械工程—光学工程] R318.51[理学—光学]