星状神经节阻滞缓解睡眠剥夺大鼠海马兴奋性毒性与空间学习记忆损伤  

Stellate ganglion block alleviates hippocampal excitotoxicity and spatial learning memory impairment in sleep-deprived rats

作　　者：郑碧琼 李佳琦 刘昌毅 郑如洁 胡淑辉[4] ZHENG Biqiong;LI Jiaqi;LIU Changyi;ZHENG Rujie;HU Shuhui(Department of Anesthesiology,the First Affiliated Hospital,Fujian Medical University,Fuzhou 350004;The First Clinical Medical College of Fujian Medical University,Fuzhou 350004;Department of Urology,the First Affiliated Hospital,Fujian Medical University,Fuzhou 350004;Department of Anesthesiology,the First Affiliated Hospital of Xiamen University,Xiamen 363001,China)

机构地区：[1]福建医科大学附属第一医院麻醉科,福州350004 [2]福建医科大学附属第一医院滨海院区国家区域医疗中心麻醉科,福州350212 [3]福建医科大学附属第一医院泌尿外科,福州350004 [4]厦门大学附属第一医院麻醉科,厦门363001

出　　处：《神经解剖学杂志》2024年第4期429-434,共6页Chinese Journal of Neuroanatomy

基　　金：福建省自然科学基金(2023J01593);福建省科技创新联合资金项目(2021Y9127);福建省中青年教师教育科研项目(JAT210114)。

摘　　要：目的:观察星状神经节阻滞(SGB)对快速眼动睡眠剥夺(RSD)大鼠空间学习记忆功能减退的保护作用与可能机制。方法:32只大鼠按对照组(Control)、RSD组、SGB组、星状神经节阻滞干预的快速眼动睡眠剥夺(RSD+SGB)组随机分配。RSD组与RSD+SGB组采用改良多平台水环境法(MMPM)进行造模,SGB组与RSD+SGB组大鼠通过SGB法进行干预。选用Morris水迷宫(MWM)评价各组大鼠的空间学习和记忆能力,选用Western Blot检测各组大鼠海马组织N-甲基-D-天门冬氨酸受体1(NR1)与caspase-3的表达水平,选用比色法检测各组大鼠海马组织谷氨酸(Glu)与天门冬氨酸(Asp)的表达水平。结果:与RSD组大鼠相比,RSD+SGB组大鼠经过SGB干预后逃逸潜伏期显著缩短(P<0.05),而穿越原平台位置的次数与原象限滞留时间占比均明显增加(P<0.05);同时,海马组织Glu、Asp、NR1与caspase-3的表达水平均显著降低(P<0.05)。结论:SGB通过降低RSD大鼠兴奋性氨基酸过度活化引起的海马组织兴奋性毒性与凋亡,来保护RSD引起的空间学习记忆能力损伤。Objective:To observe the protective effect and possible mechanism of stellate ganglion block(SGB)on spatial learning memory function impairment in rapid eye movement sleep deprived(RSD)rats.Methods:Thirty-two rats were randomly assigned to Control group,RSD group,SGB group,and rapid eye movement sleep deprivation with stellate ganglion block intervention(RSD+SGB)group.The rats in RSD group and RSD+SGB group were modeled by modified multi-platform method(MMPM),and the rats in SGB group and RSD+SGB group were intervened by the SGB method.Morris water maze(MWM)was selected to evaluate the spatial learning and memory functions of rats in each group,and the expression levels of glutamate(Glu)and aspartate(Asp)in hippocampal tissues of rats in each group were detected by colorimetric assay,and the expression levels of N-methyl-D-aspartate receptor 1(NR1)and caspase-3 in hippocampal tissues of rats in each group were detected by Western Blot.Results:Compared with the RSD group,rats in RSD+SGB group had a significantly shorter escape latency after SGB intervention(P<0.05),while the number of passes through the original platform position and the percentage of target quadrant time were significantly increased(P<0.05);at the same time,the hippocampal tissues’expression levels of Glu,Asp,NR1,and caspase-3 were all significantly reduced(P<0.05).Conclusion:SGB protects against RSD-induced impairment of spatial learning memory capacity by reducing hippocampal tissue excitotoxicity and apoptosis induced by excitatory amino acid hyperactivation in RSD rats.

关 键 词：快速眼动睡眠剥夺 星状神经节阻滞 空间学习记忆功能 兴奋性氨基酸 大鼠 

分 类 号：R614[医药卫生—麻醉学]