菊花脑PYL基因家族的全基因组鉴定及表达分析  

Genome-wide Identification and Expression Analysis of PYL Gene Family in Chrysanthemum nankingense

作　　者：郑重玖 宋孜文 杨俊俊 张兵 艾鹏慧[2] ZHENG Chongjiu;SONG Ziwen;YANG Junjun;ZHANG Bing;AI Penghui(Kaifeng Landscaping Center,Kaifeng,Henan475004,China;Henan University,Kaifeng,Henan475004,China;Puyang Academy of Agricultural and Forestry Sciences,Puyang,Henan457000,China)

机构地区：[1]开封市园林绿化中心,河南开封475004 [2]河南大学,河南开封475004 [3]濮阳市农林科学院,河南濮阳457000

出　　处：《天津农业科学》2024年第8期9-17,26,共10页Tianjin Agricultural Sciences

基　　金：河南省科技兴林项目(YLK202309);开封市科技发展计划项目(2303142);开封市农业类科技攻关项目(2302006)。

摘　　要：PYL(Pyrabactin resistance 1-like)蛋白作为脱落酸信号转导的核心组件,在植物生长发育和逆境胁迫响应中起到重要作用。为探究菊花脑PYL家族的功能,利用生物信息学方法鉴定菊花脑(Chrysanthemum nankingense)PYL基因家族成员,并利用RNA-Seq数据分析CnPYLs在不同组织中的表达情况。结果表明:CnPYL家族包括11个成员,分为3个亚家族,同一个亚家族的各成员蛋白结构域和基因结构高度保守,其中亚家族I基因含有2个内含子,其他2个亚家族基因没有内含子;CnPYL家族基因启动子区域顺式作用元件主要包括逆境胁迫响应元件、植物生长发育相关元件、光响应元件和激素响应元件。同时,RNA-Seq数据分析表明,PYL基因在菊花脑中的表达具有组织特异性,除CnPYL2基因在根中的表达量较低外,其他CnPYLs基因在菊花脑各组织中的表达都很高。这说明PYL作为ABA受体成员,参与了ABA调控的菊花脑各组织的生长发育过程。综上,本研究结果为深入研究CnPYLs基因的功能提供了基础信息。PYL(Pyrabactin resistance 1-like)protein plays an irreplaceable role,that plays an important role in plant growth,development and stress.In order to explore the function of PYL gene family in Chrysanthemum nankingense,this study identified PYL gene family members in Chrysanthemum nankingense using a bioinformatic methods,and the expression of CnPYLs in different tissues was analysed using RNA-Seq data.The results showed that the CnPYL gene family consists of 11 members,which could be divided into 3 subfamilies,and protein domains and gene structures were highly conserved of the same subfamily,the subfamily I gene contained two introns and the other two subfamily genes had no introns.Cis-acting elements analysis found that PYL family genes mainly contained elements related to stress,growth,light and hormone.The cis-acting elements in the promoter region of CnPYL family genes mainly included adversity stress-responsive elements,plant growth and development-related elements,light-responsive elements and hormone-responsive elements.RNA-Seq data analysis showed that the expression of PYL gene in Chrysanthemum nankingense was tissue-specific,exception of the CnPYL2 gene,which was expressed at a relatively low level in roots,indicating that these genes might play an essential role in the ABA-regulated growth and development of various tissues of the Chrysanthemum nankingense.The results of this study lay a theoretical foundation for further elucidating the function of CnPYLs gene in Chrysanthemum nankingense.

关 键 词：PYL 基因家族 生物信息学 菊花脑 基因表达 

分 类 号：S682.11[农业科学—观赏园艺]