麝香接骨胶囊中龙血竭替代血竭投料的研究  

Study on the Substitution of Resina Draconis for Draconis Sanguis in Shexiang Jiegu Capsules

作　　者：邓茂芝 吴喆 周云峰 邓杰华 DENG Maozhi;WU Zhe;ZHOU Yunfeng;DENG Jiehua(Yichun Inspection and Testing Center,Yichun 336000,China)

机构地区：[1]宜春市检验检测中心,江西宜春336000

出　　处：《江西中医药大学学报》2024年第5期74-78,共5页Journal of Jiangxi University of Chinese Medicine

基　　金：宜春市科学技术局指导性计划科研项目(2022ZDJH6029)。

摘　　要：目的:以龙血素A、龙血素B为专属性目标,建立麝香接骨胶囊中龙血竭替代血竭投料的鉴别研究方法。方法:采用HPLC-UV法对处方制剂3%磷酸甲醇溶液提取液进行定性分析,再以UPLC-MS/MS法进行定性验证。结果:建立的HPLC-UV法测定龙血素A、龙血素B,线性较好,回收率分别为98.23%,98.81%,RSD分别为1.41%,0.89%;UPLC-MS/MS法专属性好,灵敏度高,能够准确验证检出。此外,经分析测定,26批样品中有6批检出龙血素A、龙血素B成分,不合格率23%,表明制剂存在血竭原料被龙血竭掺伪的情况。结论:该方法简便、快速、准确,可用于麝香接骨胶囊中龙血竭替代血竭投料的检查分析。Objective:Take loureirin A and loureirin B as specific targets,to establish a detection method to identify the substitution of resina draconis for draconis sanguis in Shexiang Jiegu Capsules.Methods:Qualitative analysis of the 3%phosphoric acid methanol solution extract of the prescription formulation was performed using HPLC-UV method,and then qualitative validation was performed using UPLCMS/MS method.Results:The established HPLC-UV method for the determination of loureirin A and loureirin B showed good linearity,with recoveries of 98.23%and 98.81%,and RSDs of 1.41%and 0.89%,respectively.The UPLC-MS/MS method has good specificity,high sensitivity,and can accurately verify detection.In addition,after analysis and determination,6 out of 26 batches of samples were tested for loureirin A and loureirin B components,and the unqualified rate was 23%,indicating that the raw material of draconis sanguis was mixed with fake resina draconis.Conclusion:This method is simple,fast,and accurate,and can be used for the inspection and analysis of draconis sanguis as a substitute for resina draconis in Shexiang Jiegu Capsules.

关 键 词：麝香接骨胶囊 龙血竭 血竭 龙血素A 龙血素B 

分 类 号：R286.0[医药卫生—中药学]