云南省某乌骨鸡种鸡场鸡白痢沙门氏菌分子生物学特性研究  

Study on molecular biological characterization of Salmonella pullorum from a breeding farm of Black-Boned chicken in Yunnan Province

作　　者：高感 张诗诗 武书杰 赵勇 木卫武 向斌 和凤平 杨亮宇 张立梅[1,2] GAO Gan;ZHANG Shishi;WU Shujie;ZHAO Yong;MU Weiwu;XIANG Bin;HE Fengping;YANG Liangyu;ZHANG Limei(College of Veterinary Medicine,Yunnan Agricultural University,Kunming 650201,China;Center for Poultry Disease Control and Prevention,Yunnan Agricultural University,Kunming 650201,China;Nujiang Animal Husbanbry Technology Extension Station,Nujiang 673299,China)

机构地区：[1]云南农业大学动物医学院,昆明650201 [2]云南农业大学家禽疾病防控研究中心,昆明650201 [3]怒江州畜牧技术推广站,云南怒江673299

出　　处：《黑龙江畜牧兽医》2024年第19期55-61,68,120,共9页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家重点研发计划项目(2022YFD1601900)。

摘　　要：为了明确云南省某乌骨鸡种鸡场鸡白痢沙门氏菌的分子生物学特性,试验收集了90份18~21日龄的未出壳死胚样品和100份下痢鸡的泄殖腔棉拭子,采用沙门氏菌属血清诊断试剂盒和PCR方法进行病原菌的分离鉴定,并对分离菌进行MLST分型、药敏试验、毒力基因和耐药基因检测。结果表明:在90份未出壳死胚样品中共分离到80株鸡白痢沙门氏菌(88.89%,80/90),而泄殖腔棉拭子样品中未分离到鸡白痢沙门氏菌。80株鸡白痢沙门氏菌均为ST92型(100%,80/80);均携带invA、hilA、sefA、sipA、sipC、sopB、sopE、spvC、ssaR、ssrA、stn P1等11种毒力基因,均未检出毒力基因pef A和rck;对红霉素的耐药率最高,为93.75%(75/80),而对氧氟沙星、庆大霉素、氟苯尼考、多黏菌素B、多西环素、加替沙星、氨苄西林、阿莫西林、头孢西丁、头孢他啶、链霉素、四环素和复方新诺明均敏感;均未检出耐药基因,与耐药表型一致。说明从云南省某乌骨鸡种鸡场分离到的鸡白痢沙门氏菌来源单一,80株鸡白痢沙门氏菌均携带11种毒力基因,提示其毒力较强;除了红霉素外,对其他药物均敏感,提示该乌骨鸡种鸡场用药相对安全。In order to clarify molecular biological characterization of Salmonella pullorum from a breeding farm of Black-Boned chicken in Yunnan Province,in the test,ninety samples of unembryonic dead embryos from 18 to 21 days of age and 100 cloacal swabs of chickens with dysentery were collected;Salmonella sero-diagnostic kit and PCR method were used for the isolation and identification of pathogenic bacteria;and the isolates were subjected to carry out MLST typing,drug susceptibility test,and detection of virulence genes and drug resistance genes.The results showed that a total of 80 strains of Salmonella pullorum(88.89%,80/90)were isolated from 90 samples of unembryonated dead embryos;whereas Salmonella pullorum was not isolated from cloacal swab samples.All 80 strains of Salmonella pullorum were ST92(100%,80/80);all carried invA,hilA,sefA,sipA,sipC,sopB,sopE,spvC,ssaR,ssrA,and stnP1,for a total of 11 virulence genes;none of them had detectable virulence genes pefA and rck.The highest resistance rate was 93.75%(75/80)to erythromycin;whereas it was sensitive to ofloxacin,gentamicin,florfenicol,polymyxin B,doxycycline,gatifloxacin,ampicillin,amoxicillin,cefoxitin,ceftazidime,streptomycin,tetracycline,and cotrimoxazole;all of them did not have any detectable resistance genes,which was in line with the resistance phenotype.The results indicated that Salmonella pullorum isolated from a breeding farm in Yunnan Province was from a single source;all 80 strains of Salmonella pullorum carried 11 virulence genes,suggesting strong virulence;they were sensitive to all drugs except erythromycin,suggesting that it was relatively safe to use drugs in this farm.

关 键 词：乌骨鸡 鸡白痢沙门氏菌 分离 鉴定 毒力基因 耐药表型 

分 类 号：S831.7[农业科学—畜牧学] S852.612[农业科学—畜牧兽医]