五知散对免疫应激雏鸡生长性能与免疫功能的影响  

Effects of Wuzhisan on growth performance and immune function of immune-stressed chicks

作　　者：郑晓漫 孙晗 林咏诗 奚凯伦 高雲 郑悉睿 郭世宁[1,2] 石达友 武力[1,2] 刘翠 ZHENG Xiaoman;SUN Han;LIN Yongshi;XI Kailun;GAO Yun;ZHENG Xirui;GUO Shining;SHI Dayou;WU Li;LIU Cui(Institute of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;Guangdong Technology Research Center for Traditional Chinese Veterinary Medicine and Nature Medicine,Guangzhou 510642,China)

机构地区：[1]华南农业大学兽医学院,广州510642 [2]广东省兽用中药与天然药物工程技术研究中心,广州510642

出　　处：《黑龙江畜牧兽医》2024年第19期76-86,121,共12页Heilongjiang Animal Science And veterinary Medicine

基　　金：2022年广东省企业科技特派员专项“中药饲料添加剂的研制”。

摘　　要：为了探究自拟中药复方五知散对免疫应激雏鸡生长性能与免疫功能的影响,试验基于网络药理学与分子对接技术初步探究五知散缓解鸡免疫应激的作用机制,通过TCMSP数据库筛选五知散中五味子、金银花、知母、甘草的活性成分,在TCMSP数据库获得相对应的靶点;通过在线软件Venny 2.1获取五知散和鸡免疫应激的交集靶点,预测五知散缓解鸡免疫应激的靶点;采用David数据库和微生信平台对五知散缓解鸡免疫应激相关靶点进行GO功能富集分析与KEGG通路分析;将蛋白互作分析网络图中度值排名前两位的靶点及其对应的活性成分通过Autodocktools 1.5.7软件进行分子对接。将200只雏鸡随机分为5组,每组4个重复,每个重复10只。1日龄起,空白组、模型组饲喂基础日粮,五知散高、中、低剂量组分别在日粮中添加2%(20 g/kg)、1%(10 g/kg)、0.5%(5 g/kg)复方五知散,在应激I期(试验第21天)、应激Ⅱ期(试验第35天)检测免疫应激雏鸡生产性能指标、脏器免疫指数、血清生化指标、血清炎症因子。结果表明:五知散含槲皮素、木犀草素、山奈酚及甘草查尔酮A等114个活性成分,可作用于B细胞淋巴瘤2(Bcl-2)、白细胞介素-6(IL-6)等75个靶点,影响病灶粘连、MAPK信号通路、细胞凋亡等相关通路,其核心靶点与主要活性成分的对接结合能均<-20.83 k J/mol,有较好的结合活性。与模型组相比,在试验22~28 d,五知散高、低剂量组平均日采食量(ADFI)显著提高(P<0.05);试验29~35 d,平均日增重(ADG)显著提高(P<0.05);试验第21天,五知散中、低剂量组血清内毒素(ET)活性显著或极显著降低(P<0.05或P<0.01),低剂量组血清皮质酮(CORT)浓度显著降低(P<0.05),血清Bcl-2浓度极显著降低(P<0.001);试验第35天,五知散高剂量组胸腺和法氏囊指数极显著升高(P<0.01),血清总蛋白(TP)浓度极显著升高(P<0.01),血糖(GLU)浓度显著升高(P<0.05),肿瘤坏死因子-α(TNIn order to investigate the effects of Wuzhisan(WZS),a self-formulated traditional Chinese medicine compound,on the growth performance and immune function of immune-stressed chicks,the mechanism of WZS in relieving chickens'immune stress was preliminary explored based on network pharmacology and molecular interworking technology in the experiment.The active ingredients of Schisandra chinensis,Lonicera japonica Thunb,Anemarrhenae Rhizom,and Glycyrrhiza glabra in WZS were screened through the TCMSP database,and the corresponding targets were obtained in the TCMSP platform.The intersection targets of WZS and chicken immune stress were predicted by the online software Venny 2.1,and the targets of WZS alleviating chicken immune stress were predicted.GO functional enrichment analysis and KEGG pathway analysis were performed on the targets related to WZS alleviating chicken immune stress by the database of David and the platform of Microbiology.The targets at the top two rankings in the network diagram of the protein interactions and their corresponding active ingredients were docked by Autodocktools1.5.7 software.Two hundred chicks were randomly divided into five groups with four replicates per group and 10 chicks per replicate.From 1 day of age,animals in the blank and model groups(high,medium and low dose groups of WZS)were fed the basal diets and the basal diet supplemented with 2%compound WZS(20 g/kg),1%compound WZS(10 g/kg),and 0.5%compound WZS(5 g/kg),respectively.At the stage of stressⅠ(day 21 of the experiment)and stressⅡ(day 35 of the experiment),the production performance indexes,organ immunity indexes,serum biochemical indexes,and serum inflammatory factors of the chicks under immune stress were detected.The results showed that WZS contained 114 active ingredients,including quercetin,luteolin,kaempferol and licochalcone A,which could act on 75 targets,including B-cell lymphoma 2(Bcl-2)and IL-6,and affect the focal adhesion,MAPK signaling pathway,apoptosis and other related pathways.The docking binding en

关 键 词：五知散 免疫应激 网络药理学 生长性能 免疫功能 

分 类 号：S859.3[农业科学—临床兽医学]