噬菌体防治细菌生物被膜相关感染的研究进展  

Research progress of phages in prevention and treatment of bacterial biofilm-associated infections

作　　者：贺丽莎 杨灼 冉蕾 陈红伟[1,2,3,4] 杨洪早 HE Lisha;YANG Zhuo;RAN Lei;CHEN Hongwei;YANG Hongzao(College of Veterinary Medicine,Southwest University,Chongqing 402460,China;National Center of Technology Innovation for Pigs,Chongqing 402460,China;Immunology Research Center,Medical Research Institute,Southwest University,Chongqing 402460,China;Traditional Chinese Veterinary Research Institute,Southwest University,Chongqing 402460,China)

机构地区：[1]西南大学动物医学院,重庆402460 [2]国家生猪技术创新中心,重庆402460 [3]西南大学医学研究院,免疫学研究中心,重庆402460 [4]西南大学,中兽医药研究所,重庆402460

出　　处：《微生物学报》2024年第10期3670-3684,共15页Acta Microbiologica Sinica

基　　金：国家生猪技术创新中心先导科技项目(NCTIP-XD/B12,NCTIP-XD/C17);中央高校基本科研业务费(SWU-KQ22045);重庆市技术创新与应用发展专项面上项目(CSTB2023TIAD-LDX0006);鲁渝科技协作项目(2022LYXZ030);云南省科技厅重点研发计划(202403AC100013)。

摘　　要：细菌生物被膜是由细菌产生的胞外多聚物围绕而成的聚集体,其对抗菌剂和宿主免疫防御机制展现出显著抗性,成为临床细菌感染疾病难以根治的关键因素。噬菌体作为一类能够特异性感染并裂解细菌的病毒,在防治生物被膜相关感染领域展现出巨大潜力。本文综述了噬菌体、噬菌体内溶素以及噬菌体协同抗生素防治生物被膜相关感染的作用效果、体内外研究方法与作用机理,并探讨了噬菌体在防治生物被膜相关感染中的前景与潜在障碍,同时进行展望,旨在为开发更高效的治疗药物提供有价值的参考。Bacterial biofilms are aggregates surrounded by extracellular polymeric substances produced by bacteria,exhibiting significant resistance to antimicrobials and host immune defense mechanisms.As a result,they become a crucial factor in the recalcitrance of bacterial infections.Phages,as a class of viruses capable of specifically infecting and lysing bacteria,have demonstrated immense potential in the prevention and treatment of biofilm-associated infections.This review summarizes the efficacy,in vitro and in vivo research methods,and mechanisms of phages,phage endolysins,and phage-antibiotic combinations in combating biofilm-associated infections.Furthermore,it discusses the prospects and potential obstacles of phages in this field,aiming to give insights into the development of more effective therapeutic agents.

关 键 词：噬菌体 细菌生物被膜 内溶素 噬菌体-抗生素联合疗法 

分 类 号：Q939.9[生物学—微生物学]