吉林地区朝、满、汉3个民族KIR基因的分布频率研究  

Distribution frequencies of KIR genes among the Korean,Manchu and Han ethnic groups in Jilin Province

作　　者：韩瑜[1] 杨帆[1] 刘玲玲 聂婷婷 杨旭 焦立新[1] HAN Yu;YANG Fan;LIU Lingling;NIE Tingting;YANG Xu;JIAO Lixin(Institution of Blood Transfusion,Changchun Central Blood Station,Changchun 130033,China)

机构地区：[1]长春市中心血站输血研究所,吉林长春130033

出　　处：《中国输血杂志》2024年第10期1132-1136,共5页Chinese Journal of Blood Transfusion

基　　金：吉林省卫生健康科技能力提升项目(2022JC026)。

摘　　要：目的通过分析吉林地区汉族、满族及朝鲜族的杀伤细胞免疫球蛋白样受体(KIR)基因的频率与多态性,为探究KIR基因与疾病关联提供基础数据支持。方法采用聚合酶链反应-序列特异性引物(PCR-SSP)技术对吉林地区129名满族、198名朝鲜族及201名汉族人群的KIR基因进行分型。结果KIR3DL2、KIR3DL3、KIR3DP1及KIR2DL4在所有检测对象中均为100%检出。KIR2DL1、KIR2DL3、KIR2DS4、KIR3DL1及KIR2DP1等基因在这3个民族中的检出频率较高,介于93%~98%之间。相比之下,KIR2DL2、KIR2DL5、KIR3DS1、KIR2DS1、KIR2DS2、KIR2DS3及KIR2DS5的检出率较低,分布在13%~45%。特别是满族KIR2DL5(17.83%)与KIR2DS1(17.83%)的基因检出频率显著低于吉林地区朝鲜族(42.93%、47.47%)和汉族(33.83%、33.33%);吉林地区朝鲜族KIR2DL5(42.93%)与KIR2DS1(47.47%)的检出频率显著高于汉族(33.83%、33.33%)和满族(17.83%、17.83%);吉林地区汉族单体型KIRAA的频率在吉林3个受检民族中是最高的为61.19%,显著高于朝鲜族(42.93%),以上各组差异比较P<0.05,经Bonferroni校正后,Pc<0.05。结论吉林地区的朝鲜族、满族与汉族在KIR基因分布方面既体现了中国人群KIR基因多态性,又各自展示了独特的民族遗传特性和地域性。Objective To provide foundational data for exploring the association between KIR genes and diseases by analyzing the frequency and polymorphism of killer-cell immunoglobulin-like receptor(KIR)genes in Han,Manchu and Korean populations in Jilin Province.Methods KIR gene typing was performed on 129 Manchu,198 Korean and 201 Han individuals from Jilin using the polymerase chain reaction-sequence specific primers(PCR-SSP)technique.Results KIR3DL2,KIR3DL3,KIR3DP1 and KIR2DL4 were detected in all subjects.KIR2DL1,KIR2DL3,KIR2DS4,KIR3DL1 and KIR2DP1 genes had high detection frequencies,ranging from 93%to 98%across the three ethnic groups.In contrast,the detection rates of KIR2DL2,KIR2DL5,KIR3DS1,KIR2DS1,KIR2DS2,KIR2DS3 and KIR2DS5 were lower,ranging from 13%to 45%.Notably,the detection frequencies of KIR2DL5(17.83%)and KIR2DS1(17.83%)in the Manchu population were significantly lower than those in the Korean(42.93%,47.47%)and Han(33.83%,33.33%)populations in Jilin.The detection frequencies of KIR2DL5(42.93%)and KIR2DS1(47.47%)were significantly higher in the Korean population compared to the Han(33.83%,33.33%)and Manchu(17.83%,17.83%)population.The frequency of the KIRAA haplotype in the Han population was the highest among the three ethnic groups in Jilin at 61.19%,significantly higher than that in the Korean population(42.93%).Differences between the three groups were statistically significant(P<0.05),and remained significant after Bonferroni correction(Pc<0.05).Conclusion The distribution of KIR genes in the Korean,Manchu and Han population in Jilin reflects the polymorphism of KIR genes in the Chinese population and also showcases unique ethnic genetic and regional characteristics,which enhances our understanding of KIR gene diversity and ethnic specificity.

关 键 词：杀伤细胞免疫球蛋白样受体 基因多态性 基因型 朝鲜族 满族 

分 类 号：Q343.14[生物学—遗传学]