乳液静电纺制备PCL/TCH图案化纳米纤维膜  

Preparation of PCL/TCH patterned nanofiber membranes by emulsion electrospinning

作　　者：章军 余景红 李庭晓 辛斌杰[1] ZHANG Jun;YU Jinghong;LI Tingxiao;XIN Binjie(School of Textiles and Fashion,Shanghai University of Engineering Science,Shanghai 201620,China;College of Textiles,Donghua University,Shanghai 201620,China)

机构地区：[1]上海工程技术大学纺织服装学院,上海201620 [2]东华大学纺织学院,上海201620

出　　处：《现代纺织技术》2024年第10期20-30,共11页Advanced Textile Technology

基　　金：国家自然科学青年基金项目(2041808165)。

摘　　要：为解决药物突释问题,实现药物的有效包埋和长效释放,设计了具有稳定皮芯结构的图案化微纳米纤维膜,用于药物控释领域。采用乳液静电纺丝技术,将盐酸四环素(TCH)作为模型药物,以聚己内酯(PCL)/六氟异丙醇(HFIP)作为油相、TCH/水溶液作为水相进行纺丝并优化工艺参数,使盐酸四环素(TCH)在纺丝过程中保持性能稳定并能够持续释放。同时,探究图案化对纳米纤维膜的力学性能、载药能力和TCH在体外释放性能的影响。结果表明:当药物质量分数为2%时,纺丝乳液的液滴均匀性较好,液滴的平均直径为0.65μm,制备的纤维形貌均匀连续,平均直径为200 nm,药物累计释放率达到了52.15%;图案化纤维膜具有良好的柔韧性和延展性,断裂伸长率从78.65%提高到98.96%,载药能力和TCH在体外释放性能均有所提升,药物累计释放率从52.15%提高到65.60%。该研究表明,通过乳液静电纺丝技术,可以将模型药物TCH包裹在PCL的内部,有效改善PCL/TCH纤维膜的药物释放性能,同时图案化可进一步提高机械性能。因此,通过乳液静电纺制备的PCL/TCH图案化纳米纤维膜在医疗敷料方面具备一定的应用前景。With the continuous innovation and vigorous development of advanced textile technology new technologies such as emulsion electrostatic spinning coaxial electrostatic spinning and patterning are gaining popularity.In the field of drug delivery the nanofiber membrane made by the traditional electrostatic spinning technology as a drug carrier has certain limitations such as the serious phenomenon of sudden drug release uneven distribution of drugs in the fiber and so on.In order to alleviate the phenomenon of sudden drug release a nanofibrous membrane with a skin-core structure shall be prepared as a solution to effectively encapsulate the drug and maintain the biological activity of the drug molecules by taking advantage of the fact that the core layer of the fiber is not in contact with the outside world.Currently emulsion electrostatic spinning and coaxial electrostatic spinning can prepare nanofibrous membrane with skin-core structure.Compared with coaxial electrospinning emulsion electrostatic spinning is simple to operate and the encapsulation of different drugs/active molecules can be achieved by water-in-oil or oil-in-water.Meanwhile by patterning to mimic the multi-layered structure of the extracellular matrix ECM not only the surface area of the fibrous membrane can be increased to improve its drug release properties but also the orientation of the fibers can be altered to improve the ability of cell adhesion growth and differentiation.In this paper patterned nanofibrous membranes with polycaprolactone PCL as the skin layer and tetracycline hydrochloride TCH as the core layer were prepared by emulsion electrostatic spinning.Firstly a certain amount of PCL was dissolved in 1 mL of hexafluoroisopropanol HFIP and then 19.88 mg of Span80 was added as an emulsifier and stirred thoroughly and afterwards aqueous solutions of TCH at various concentrations were introduced into the stirring PCL oil-phase solution creating a mixture with a 6%aqueous-oil phase volume ratio.In the final step the PCL/TCH patterned nanof

关 键 词：聚己内酯 盐酸四环素 乳液静电纺 图案化 药物控释 

分 类 号：TQ340.64[化学工程—化纤工业]