淡水和海洋沉积物中N-DAMO古菌对聚氯乙烯微塑料的响应研究  

Response of N-DAMO Archaea to Polyvinyl Chloride Microplastics in Freshwater and Marine Sediments

作　　者：有海荣 刘帅 You Hairong;Liu Shuai(College of Environment and Safety Engineering,Qingdao University of Science and Technology,Qingdao 266000,China)

机构地区：[1]青岛科技大学环境与安全工程学院,山东青岛266000

出　　处：《山东化工》2024年第18期44-48,共5页Shandong Chemical Industry

基　　金：山东省自然科学基金项目(ZR2021MD037)。

摘　　要：硝酸盐型厌氧甲烷氧化(Nitrate-dependent anaerobic methane oxidation,N-DAMO)对自然环境中的甲烷减排起着重要作用。近年来,由于人类活动的影响,自然环境中的微塑料含量逐渐增加。然而,微塑料对N-DAMO古菌的生态分布及功能的影响尚不清楚,微塑料在甲烷减排中的作用有待探究。本研究以淡水和海洋沉积物为对象,利用同位素示踪技术和分子生物学手段研究了不同聚氯乙烯微塑料浓度处理下沉积物中N-DAMO古菌的活性,丰度及多样性。结果表明:聚氯乙烯微塑料在短期内显著提高淡水和海洋沉积物N-DAMO古菌活性。聚氯乙烯微塑料对海洋沉积物中的N-DAMO古菌活性刺激更强。加入微塑料后,淡水和海洋沉积物的N-DAMO古菌多样性均发生改变,海洋沉积物中的N-DAMO古菌群落结构更容易受到微塑料影响。Nitrate-dependent anaerobic methane oxidation(N-DAMO)plays an important role in methane emission reduction in natural environment.In recent years,due to the influence of human activities,the content of microplastics(MPs)in the natural environment had gradually increased.However,the effects of MPs on the ecological distribution and function of N-DAMO archaea remain unclear,and the role of MPs in methane emission reduction remains to be explored.In this study,we examined the activity,abundance,and diversity of N-DAMO archaea in freshwater and marine sediments under different concentrations of polyvinyl chloride MPs using isotope tracing techniques and molecular biology methods.Results showed that polyvinyl chloride MPs significantly increased the activity of N-DAMO archaea in freshwater and marine sediments in the short term.polyvinyl chloride MPs were more effective in stimulating the activity of N-DAMO archaea in marine sediments.The diversity of N-DAMO archaea in both freshwater and marine sediments changed after the addition of MPs,and the community structure of N-DAMO archaea in marine sediments was more susceptible to MPs.

关 键 词：厌氧甲烷氧化 N-DAMO古菌 海洋生态系统 淡水生态系统 微塑料污染 

分 类 号：X52[环境科学与工程—环境工程] X55