黄蜀葵花药材总多糖含量测定方法研究  

Study on the Determination of Polysaccharide Content in Abelmoschi Corolla

作　　者：张清华 陈玲芳[2] 郭小藤 蒋露 尚立霞 Zhang Qinghua;Chen Lingfang;Guo Xiaoteng;Jiang Lu;Shang Lixia(Shandong Academy of Pharmaceutical Science,Jinan 250101,China;Hangzhou Conba pharmaceutical Co.,Ltd.,Hangzhou 310052,China)

机构地区：[1]山东省药学科学院,山东济南250101 [2]杭州康恩贝制药有限公司,浙江杭州310052

出　　处：《山东化工》2024年第18期136-138,共3页Shandong Chemical Industry

基　　金：浙江省“尖兵”“领雁”研发攻关计划资助(编号:2023C04049)。

摘　　要：目的:建立黄蜀葵花药材中总多糖的含量测定方法,为其质量控制提供依据。方法:以葡萄糖为对照,用苯酚-硫酸法对样品显色,采用紫外-可见分光光度法在485 nm波长处测定总多糖的含量,并对方法学进行研究。结果:总多糖在2.270~13.620μg/mL范围内呈良好线性关系;其回归方程为Y=0.0612186X-0.0178058(r=0.999),加样回收率为99.15%,RSD为0.66%(n=9)。结论:采用苯酚-硫酸法测定黄蜀葵花药材中总多糖的含量,方法稳定,精密度高,重复性好,适用于黄蜀葵花药材的多糖含量测定。Objective:To establish the method to deteminate the content of polysaccharide in Abelmoschi Corolla,it may provide the basis for quality control of Abelmoschi Corolla.Methods:Phenol-sulfu-ricacid method was used for chromogenic recction.The content of total polysaccharide was measured by UV spectrophotometry at 485 nm.Results:The total polysaccharides calibration curve was at the range of 2.270~13.620μg/mL,with regression function being Y=0.0612186X-0.0178058(r=0.999).The recowery of calycosin was 99.15%,RSD为0.66%(n=9).Conclusion:The phenolsulfuric acid method is stable,reliable,simple and easy to operate in the determination of polysaccharides in Abelmoschi Corolla.

关 键 词：黄蜀葵花 总多糖 苯酚-硫酸法 紫外-可见分光光度法 含量测定 

分 类 号：TQ461[化学工程—制药化工]