基于银纳米片的形貌定向演化比色检测药物硫普罗宁  

Colorimetric Detection of Tiopronin Based on Morphology Evolution of Silver Nanoplates

作　　者：王燚[1] 秦冬玲 孙琴 WANG Yi;QIN Dongling;SUN Qin(Chongqing Key Laboratory of Green Catalysis Materials and Technology,College of Chemistry,Chongqing Normal University,Chongqing 401331,China)

机构地区：[1]重庆师范大学化学学院绿色催化材料与技术重庆市重点实验室,重庆401331

出　　处：《重庆师范大学学报（自然科学版）》2024年第4期42-49,共8页Journal of Chongqing Normal University:Natural Science

基　　金：重庆市高校创新研究群体项目(No.CXQT21015);重庆师范大学博望学者领军人才项目(No.BWLJ2023009)。

摘　　要：为了更加准确、快速、简便地检测用于治疗肝脏疾病的药物硫普罗宁(tiopronin,MPG),建立了一种基于三角形银纳米片(AgNPRs)的形貌变化来调控它的局域表面等离子体共振吸收(LSPR)进而实现比色测定MPG含量的新方法。由于AgNPRs尖角部分表面能量较高且会被一定浓度的Cl-氧化蚀刻,因此它的微观形貌由三角片转化为尺寸更小的圆片,相应的LSPR吸收带发生明显蓝移;MPG与AgNPRs共存时,由于前者分子结构中的巯基与Ag之间存在特异性作用,故能发挥保护功能使后者不易被Cl-蚀刻,从而控制后者LSPR吸收带不发生位移。所建立的可视化检测MPG的比色分析新方法对MPG的检测范围为0.01~0.5μmol·L^(-1);将该方法应用于MPG注射液和MPG肠溶片中MPG含量的测定,检测结果与标示量无明显差异,加标回收率为95.3%~102.5%。该方法未来有望应用于MPG相关药品的质量控制。A new colorimetric method for the detection of tiopronin,a drug used to treat liver disease,was developed based on the morphological changes of triangular silver nanoprisms(AgNPRs)to modulate their local surface plasmon resonance(LSPR)absorption.Due to the high surface energy of the sharp corners of AgNPRs,they are easily etched by a certain concentration of Cl~-.As a result,the morphology of AgNPRs changes from triangular flakes to smaller circular flakes,and the corresponding LSPR absorption bands are significantly blue-shifted.When thiopronin coexists with AgNPRs,due to the special interaction between the thiol group of thiopronin and Ag,thiopronin plays an important role in protecting AgNPRs from Cl~-etching,thus controlling the LSPR absorption bands of AgNPRs from being shifted.Based on this principle,a new colorimetric method for the visual detection of thiopronin was developed with a detection range of 0.01~0.5μmol·L~(-1),which has been applied to the detection of thiopronin in injections and enteric-coated tablets,and the results showed that there was no significant difference from the labeled amount with the recoveries of 95.3%~102.5%.

关 键 词：银纳米粒子 贵金属纳米晶 氧化蚀刻 硫普罗宁 

分 类 号：O657[理学—分析化学]