Efficient conversion of aromatic and phenylpropanoid alcohols to acids by the cascade biocatalysis of alcohol and aldehyde dehydrogenases  

作　　者：Zetian Qiu Xiaohui Liu Jie Yu Yushuo Zhao Guang-Rong Zhao Shengying Li Kun Liu Lei Du Li Ma 

机构地区：[1]State Key Laboratory of Microbial Technology,Shandong University,Qingdao,Shandong,266237,China [2]Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),School of Chemical Engineering and Technology,Tianjin University,Yaguan Road 135,Jinnan District,Tianjin,300350,China [3]School of Health Management,Hengxing University,Qingdao,Shandong,266100,China

出　　处：《Synthetic and Systems Biotechnology》2024年第2期187-195,共9页合成和系统生物技术（英文）

基　　金：This work was supported by the National Key Research and Development Program of China(2021YFA0911500);the National Natural Science Foundation of China(32071266,32170088,and 32370032);the Shandong Provincial Natural Science Foundation(ZR2020ZD23);the State Key Laboratory of Microbial Technology Open Projects Fund(M2022-01).

摘　　要：Benzyl and phenylpropanoid acids are widely used in organic synthesis of fine chemicals,such as pharmaceuticals and condiments.However,biocatalysis of these acids has received less attention than chemical synthesis.One of the main challenges for biological production is the limited availability of alcohol dehydrogenases and aldehyde dehydrogenases.Environmental microorganisms are potential sources of these enzymes.In this study,129 alcohol dehydrogenases and 42 aldehyde dehydrogenases from Corynebacterium glutamicum,Pseudomonas aeruginosa,and Bacillus subtilis were identified and explored with various benzyl and phenylpropanoid alcohol and aldehyde substrates,among which four alcohol dehydrogenases and four aldehyde dehydrogenases with broad substrate specificity and high catalytic activity were obtained.Moreover,a cascade whole-cell catalytic system including ADH-90,ALDH-40,and the NAD(P)H oxidase LreNox was established,which showed high efficiency in converting cinnamyl alcohol and p-methylbenzyl alcohol into the respective carboxylic acids.Remarkably,this biocatalytic system can be easily scaled up to gram-level production,facilitating preparation purposes.

关 键 词：Benzyl acids Phenylpropanoid acids Alcohol dehydrogenases Aldehyde dehydrogenases Whole-cell catalysis 

分 类 号：Q81[生物学—生物工程]