The forced activation of asexual conidiation in Aspergillus niger simplifies bioproduction  

作　　者：Xingyu Wu Tingting Zhang Ke Zhang Rui Zhang Man Shi Chenlei Gu Tianqiong Shi Ling Lu Feng Xue Qing Xu Chi Zhang 

机构地区：[1]School of Food Science and Pharmaceutical Engineering,Nanjing Normal University,Nanjing,210023,China [2]College of Marine Life Sciences,Ocean University of China,Qingdao,266003,China [3]College of Life Sciences,Nanjing Normal University,Nanjing,210023,China

出　　处：《Synthetic and Systems Biotechnology》2024年第2期277-284,共8页合成和系统生物技术（英文）

基　　金：This work was financially supported by the National Key Research and Development Program of China(2021YFC2104300);the National Natural Science Foundation of China(32200055 and 22378210);the Natural Science Foundation of Jiangsu Province(BK20202002).

摘　　要：Aspergillus niger is an efficient cell factory for organic acids production,particularly l-malic acid,through genetic manipulation.However,the traditional method of collecting A.niger spores for inoculation is labor-intensive and resource-consuming.In our study,we used the CRISPR-Cas9 system to replace the promoter of brlA,a key gene in Aspergillus conidiation,with a xylose-inducible promoter xylP in l-malic acid-producing A.niger strain RG0095,generating strain brlAxylP.When induced with xylose in submerged liquid culture,brlAxylP exhibited significant upregulation of conidiation-related genes.This induction allowed us to easily collect an abundance of brlAxylP spores(>7.1×106/mL)in liquid xylose medium.Significantly,the submerged conidiation approach preserves the substantial potential of A.niger as a foundational cellular platform for the biosynthesis of organic acids,including but not limited to l-malic acid.In summary,our study offers a simplified submerged conidiation strategy to streamline the preparation stage and reduce labor and material costs for industrial organic acid production using Aspergillus species.

关 键 词：Aspergillus niger L-malic acid Submerged conidiation Simplify procedures Reduce costs 

分 类 号：Q81[生物学—生物工程]