Engineering and finetuning expression of SerC for balanced metabolic flux in vitamin B6 production  

作　　者：Kai Chen Linxia Liu Jinlong Li Zhizhong Tian Hongxing Jin Dawei Zhang 

机构地区：[1]School of Chemical Engineering and Technology,Hebei University of Technology,Tianjin,China [2]Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin,China [3]National Center of Technology Innovation for Synthetic Biology,Tianjin,China [4]Key Laboratory of Engineering Biology for Low-Carbon Manufacturing,Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin,China [5]University of Chinese Academy of Sciences,Beijing,China

出　　处：《Synthetic and Systems Biotechnology》2024年第2期388-398,共11页合成和系统生物技术（英文）

基　　金：This work was supported by the National Key R&D Program of China(2022YFC2106100);National Natural Science Foundation of China(22178372,32200049);National Science Fund for Distinguished Young Scholars(22325807);Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(TSBICIP-KJGG-011,TSBICIP-CXRC-055);Yellow River Delta Industry Leading Talents(DYRC20190212).

摘　　要：Vitamin B6 plays a crucial role in cellular metabolism and stress response,making it an essential component for growth in all known organisms.However,achieving efficient biosynthesis of vitamin B6 faces the challenge of maintaining a balanced distribution of metabolic flux between growth and production.In this study,our focus is on addressing this challenge through the engineering of phosphoserine aminotransferase(SerC)to resolve its redundancy and promiscuity.The enzyme SerC was semi-designed and screened based on sequences and predicted kcat values,respectively.Mutants and heterologous proteins showing potential were then fine-tuned to optimize the production of vitamin B6.The resulting strain enhances the production of vitamin B6,indicating that different fluxes are distributed to the biosynthesis pathway of serine and vitamin B6.This study presents a promising strategy to address the challenge posed by multifunctional enzymes,with significant implications for enhancing biochemical production through engineering processes.

关 键 词：Vitamin B6 Phosphoserine aminotransferase SerC Protein engineering Multifunctional enzymes Metabolic flux distribution 

分 类 号：Q78[生物学—分子生物学]