MEK1/2抑制剂U0126对射频消融术后残留肝癌细胞系HepG2-H作用的研究  

Study on the Effect of MEK1/2 Inhibitor U0126 on Residual Liver Cancer Cell Lines After Radiofrequency Ablation

作　　者：贾辰昊 李彬[1] 李凤娟[2] 柴虎林[1] 凌海锋 贾国群[1] JIA Chenhao;LI Bin;LI Fengjuan;CHAI Hulin;LING Haifen g;JIA Guoqun(Department of Hepatobiliary,The First Affiliated Hospital of Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014010 China;Department of Neurology,Baotou Eighth Hospital,Baotou 014040 China;Department of Laboratory,Baotou Third Hospital,Baotou 014030 China)

机构地区：[1]内蒙古科技大学包头医学院第一附属医院肝胆外科,内蒙古包头014010 [2]包头市第八医院神经内科,内蒙古包头014040 [3]包头市第三医院检验科,内蒙古包头014030

出　　处：《内蒙古医学杂志》2024年第9期1031-1034,1039,F0003,共6页Inner Mongolia Medical Journal

基　　金：内蒙古自治区自然科学基金项目(编号:2022MS08030);内蒙古自治区卫生健康科技计划项目(编号:202201416)。

摘　　要：目的研究MEK1/2抑制剂U0126对肝癌射频消融术后残留肝癌细胞系HepG2-H的增殖、迁移和侵袭的影响。方法运用肝癌细胞系HepG2体外制作射频消融术后残留肝癌细胞系HepG2-H,通过细胞增殖、划痕、侵袭实验,观察实验组(加入10μmol/L、20μmol/L、30μmol/L的U0126)和对照组(不加入U0126)细胞的增殖、迁移和侵袭能力。结果实验组(加入10、20、30μmol/L的U0126)的吸光度值分别为:(0.6929±0.08257)、(0.5084±0.06012)、(0.4549±0.07660),对照组吸光度值为:(1.1245±0.07971);组间差异有统计学意义(P<0.05);实验组的迁移面积分别为:(5640.2±285.4)mm^(2)、(5082.4±237.7)mm^(2)、(4821.7±246.3)mm^(2),对照组的迁移面积为:(6053.8±269.2)mm^(2),组间差异有统计学意义(P<0.05);实验组的每高倍视野细胞计数分别为:(164.2±18.89)个、(138.4±18.06)个、(129.4±18.54)个,对照组的每高倍视野细胞计数为:(226.6±16.81)个,组间差异有统计学意义(P<0.05)。结论U0126可抑制射频消融术后残留肝癌细胞HepG2-H的增殖、迁移和侵袭。Objective To investigate the effect of MEK1/2 inhibitor U0126 on the proliferation,migration,and invasion of residual liver cancer cell lines(HepG2-H)after radiofrequency ablation.Method HepG2 liver cancer cell line was used to prepare residual liver cancer cell line(HepG2-H)after radiofrequency ablation in vitro.Cell proliferation,scratch,and invasion experiments were conducted to observe the proliferation,migration,and invasion abilities of the experimental group(with 10μmol/L,20μmol/L,30μmol/L U0126 added)and the control group(without U0126 added)cells.Result The absorbance values of the experimental group(with 10,20,and 30μmol/L of U0126 added)were 0.6929±0.08257,0.5084±0.06012,and 0.4549±0.07660,respectively.The absorbance values of the control group were 1.1245±0.07971;There was a significant difference between groups(P<0.05);The migration areas of the experimental group were 5640.2±285.4,5082.4±237.7,and 4821.7±246.3,respectively.The migration areas of the control group were 6053.8±269.2,with significant differences between the groups(P<0.05);The cell counts per high-power field in the experimental group were 164.2±18.89,138.4±18.06,and 129.4±18.54,respectively.The cell counts per high-power field in the control group were 226.6±16.81,with significant differences between the groups(P<0.05).Conclusion U0126 can inhibit the proliferation,migration,and invasion of residual liver cancer cell lines(HepG2-H)after radiofrequency ablation.

关 键 词：肝癌细胞 射频消融 MEK1/2 U0126 

分 类 号：R657.42[医药卫生—外科学]