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作 者:李文 罗生金 张梦圆 樊新丽 LI Wen;LUO Sheng-jin;ZHANG Meng-yuan;FAN Xin-li(Hami Animal Husbandry Station,Hami,Xinjiang 839000 China)
出 处:《中国牛业科学》2024年第5期11-16,共6页China Cattle Science
基 金:新疆“天山英才”培养计划青年科技拔尖人才项目(2022TSYCJC0024);新疆乡村振兴产业发展科技行动项目“2022NC129”;新疆科技特派员项目“2022BKZ020”。;。
摘 要:为了探讨环形泰勒虫病诊断与防治效果,试验采用临床诊断、病理剖检、血液涂片染色镜检、PCR检测及硬蜱形态学鉴定等方法,针对2023年5月哈密市部分养殖户的牛出现发热、淋巴结肿大等症状,病死率达43.6%,为尽快控制病情,采集病牛血样60份,分别进行血液涂片检测和PCR诊断。结果表明:血液涂片的阳性率为55%;PCR检测阳性率为65%,进行测序比对分析发现,病原为环形泰勒虫,两种检测方法的阳性符合率为81.8%,其中养殖场的发病率高于散养户,改良牛的感染率明显高于本地牛,以2岁以下牛发病较为多见。对病死牛剖检肉眼可见,可视黏膜苍白或黄染、心脏外膜出血点、肝脏淤血、肿大,有暗红色病灶等病理变化。采集患牛体表寄生硬蜱,通过形态学鉴定确定为小亚璃眼蜱。说明针对不同病程,使用不同的处方治疗,牛环形泰勒虫病可得以控制。现提出诊断、治疗及预防措施,以期为环形泰勒虫病的防控提供参考。In order to explore the diagnosis and control effects of bovine tropical theileriosis,clinical diagnosis,pathological autopsy,blood smear staining microscopy,PCR detection,and hard tick morphological identification were used in this study.In response to the symptoms of fever and lymph node enlargement in cattle raised by some farmers in Hami City in May 2023,with a mortality rate of 43.6%,60 blood samples of diseased cattle were collected for blood smear detection and PCR diagnosis to control the disease as soon as possible.The results showed that the positive rate of blood smear PCR detection was 55%and 65%,respectively.The sequencing comparison analysis revealed that the pathogen was Theileria annulata,and the positive coincidence rate of the two detection methods was 81.8%.The incidence of scale farms had a higher morbidity rate than that of sporadic farmers,and the incidence of improved cattle was significantly higher than that of local cattle,especially cattle under 2 years old.The autopsy of dead cattle showed visible pathological changes such as pale or yellow stained mucous membranes,bleeding points on the epicardium,liver congestion,swelling,and dark red lesions.Collect parasitic hard ticks parasitizing on the surface of diseased cattle and determine them to be small glass eyed ticks through morphological identification.By using different prescriptions for different stages of disease,bovine ringworm disease can be controlled.The diagnosis,treatment,and prevention measures are proposed to provide a reference for the prevention and control of circular Taylor's disease.
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