Engineer and split an efficient hypercompact CRISPR–CasΦ genome editor in plants  

作　　者：Yan Sun Jianjian Hu Zhichao Hu Hejie Zhou Yuhong Gao Yini Liu Yuan Ji Gencheng Xu Yifan Guo Yuanyan Zhang Yunlu Tian Xi Liu Shirong Zhou Yuqiang Liu Tingdong Li Chao Li Jianmin Wan 

机构地区：[1]State Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization,Jiangsu Engineering Research Center for Plant Genome Editing,National Observation and Research Station of Rice Germplasm Resources,Nanjing Agricultural University,Nanjing 210095,China [2]Zhongshan Biological Breeding Laboratory,Nanjing 210095,China [3]Sanya Institute of Nanjing Agricultural University,Hainan Seed Industry Laboratory,Sanya 572025,China [4]State Key Laboratory of Crop Stress Biology for Arid Areas,College of Agronomy,Northwest A&F University,Yangling 712100,China [5]State Key Laboratory of Crop Gene Resources and Breeding,Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China [6]These authors contributed equally to this article

出　　处：《Plant Communications》2024年第7期8-11,共4页植物通讯（英文）

基　　金：supported by the Ministry of Agriculture and Rural Affairs of China,the Jiangsu Province Natural Science Foundation(grant nos.BK20212010 and BK20230038);the Jiangsu Province Key Research and Development Program(grant no.BE2022383);the Hainan Seed Industry Laboratory(grant no.B21HJ1004);the Zhongshan Biological Breeding Laboratory(grant no.ZSBBL-KY2023-04);the Guidance Foundation of the Sanya Institute of Nanjing Agricultural University(grant no.NAUSY-ZZ01);the Bingtuan Key Science and Technology Program of Xinjiang Province(grant no.2023AB006-02);the National Natural Science Foundation of China(grant no.31872806).

摘　　要：The programmable CRISPR-Cas genome editing technology,adopted from prokaryotic adaptive immune systems,has revolutionized genome engineering in plants(Liu et al.,2022a).Many efforts have been made to improve the activity,specificity,and protospacer adjacent motif(PAM)variants of Class 2 Cas nucleases,such as Cas9,Cas12a,and Cas12b(Liu et al.,2022a).However,their large size(∼1000–1400 amino acids)poses a challenge in scenarios requiring a compact Cas nuclease,particularly in urgent situations like plant virus-induced genome editing(Cheuk and Houde,2018;Li et al.,2021;Varanda et al.,2021).

关 键 词：CRISPR GENOME EDITOR 

分 类 号：Q78[生物学—分子生物学]