GWAS unravels acid phosphatase ACP2 as a photosynthesis regulator under phosphate starvation conditions through modulating serine metabolism in rice  

作　　者：Sushuang Liu Zhan Xu Jemaa Essemine Yanmin Liu Chundong Liu Feixue Zhang Zubair Iqbal Mingnan Qu 

机构地区：[1]Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology,Agricultural College of Yangzhou University,Yangzhou 225009,China [2]Department of Life Sciences and Health,Huzhou College,Huzhou 313000,China [3]Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China [4]Institute of Crop,Huzhou Academy of Agricultural Sciences,Huzhou 313000,China [5]These authors contributed equally to this article

出　　处：《Plant Communications》2024年第7期106-119,共14页植物通讯（英文）

基　　金：supported by the National Natural Science Foundation of China(32170245,32260447);Natural Science Foundation of Zhejiang Province(LQ20C130003);Sanya Yazhou Bay Science and Technology City(SCKJ-JYRC-2022-04);Scientific Research Fund of Zhejiang Provincial Education Department(YZ0Z145972);Huzhou Public Welfare Application Research Project(2021GZ26);National Training Programs of Innovation and Entrepreneurship for Undergraduates(2022hzxy019);Guangzhou Science and Technology Planning Project(202201010790).

摘　　要：Inorganic phosphorus(Pi)deficiency significantly impacts plant growth,development,and photosynthetic efficiency.This study evaluated 206 rice accessions from a MiniCore population under both Pi-sufficient(Pi^(+))and Pi-starvation(Pi^(-))conditions in the field to assess photosynthetic phosphorus use efficiency(PPUE),defined as the ratio of A_(sat)^(Pi^(-))to A_(sat)^(Pi^(+)).A genome-wide association study and differential gene expression analyses identified an acid phosphatase gene(ACP2)that responds strongly to phosphate availability.Overexpression and knockout of ACP2 led to a 67%increase and 32%decrease in PPUE,respectively,compared with wild type.Introduction of an elite allele A,by substituting the v5 SNP G with A,resulted in an 18%increase in PPUE in gene-edited ACP2 rice lines.The phosphate-responsive gene PHR2 was found to transcriptionally activate ACP2 in parallel with PHR2 overexpression,resulting in an 11%increase in PPUE.Biochemical assays indicated that ACP2 primarily catalyzes the hydrolysis of phosphoethanolamine and phospho-L-serine.In addition,serine levels increased significantly in the ACP2^(vBG)overexpression line,along with a concomitant decrease in the expression of all nine genes involved in the photorespiratory pathway.Application of serine enhanced PPUE and reduced photorespiration rates in ACP2 mutants under Pi-starvation conditions.We deduce that ACP2 plays a crucial role in promoting photosynthesis adaptation to Pi starvation by regulating serine metabolism in rice.

关 键 词：phosphate starvation GWAS photosynthetic adaption photosynthetic phosphate use efficiency genetic variation SNP RICE 

分 类 号：S511[农业科学—作物学]